HETEROLOGOUS CELL CONTACTS AND METABOLIC COUPLING IN BOVINE CUMULUS OOCYTE COMPLEXES
HETEROLOGOUS CELL CONTACTS AND METABOLIC COUPLING IN BOVINE CUMULUS OOCYTE COMPLEXES
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DOI：
10.1002/mrd.1080280307
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发表时间：
1991-03-01
影响因子：
2.5
通讯作者：
KRUIP, TAM
中科院分区：
文献类型：
--
作者：
DELOOS, F;KASTROP, P;KRUIP, TAM
根据牛卵丘卵母细胞复合体(COCs)的形态特征，对其卵丘细胞突起的完整性进行了研究。三种不同类型的卵丘细胞突起终末(CCPE)被识别出来，一种穿透皮质，另一种不穿透皮质，第三种形式是中间的，在外观上更为罕见。穿透皮质的突起常常与卵膜形成缝隙连接。在四个不同的COC类别中，对三种类型的CCPE的划分是针对四个类别中的三个类别的。第一类COC主要具有穿透性CCPE，第四类COC主要具有非穿透性CCPE，第二和第三类COC具有两种类型的CCPE。通过将H-3-胆碱掺入卵母细胞来评估卵丘卵母细胞接触的代谢偶联。大多数第四类COCs将低水平的胆碱转移到卵母细胞中，而其他三类卵母细胞中的大多数将高水平的胆碱转移到卵母细胞中。与其他三类相比，第四类卵母细胞在受精后能够分裂的比例较小。这种发育能力的降低可能是由于培养开始之前代谢偶联的丧失所致。
The integrity of the cumulus cell processes were studied in four categories of bovine cumulus oocyte complexes (COCs) selected on their morphological characteristics. Three different types of cumulus cell process endings (CCPEs) were identified, one penetrating the cortex, another not penetrating the cortex, and a third form was intermediate and more rare in appearance. The process endings that penetrated the cortex frequently made gap junctions with the oolemma. The division of the three types of CCPEs over the four different COC categories was specific for three of the four categories. The first-category COC predominantly possessed the penetrating CCPE, the fourth-category COC possessed predominantly the nonpenetrating CCPE, and the second and third categories had both types of CCPEs. The metabolic coupling of the cumulusoocyte contacts was assessed by means of incorporation of H-3-choline into the oocyte. The majority of category 4 COCs transferred low levels of choline into the oocyte while the majority of the oocytes of the other three categories transferred high levels of choline into the oocyte. Category 4 includes a smaller proportion of oocytes capable of cleaving after fertilization than the other three categories. This reduced developmental capacity is probably due to the loss of metabolic coupling before the onset of culture.