OBITOOLS: a UNIX-inspired software package for DNA metabarcoding
OBITOOLS: a UNIX-inspired software package for DNA metabarcoding
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DOI：
10.1111/1755-0998.12428
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发表时间：
2016-01-01
影响因子：
7.7
通讯作者：
Coissac, Eric
中科院分区：
文献类型：
--
作者：
Boyer, Frederic;Mercier, Celine;Coissac, Eric
DNA元条形码为生物多样性研究提供了新的视角。这种最近开发的生态系统研究方法在很大程度上依赖于下一代测序（NGS）的使用，因此需要处理巨大序列数据集的能力。OBITOOLS软件包满足了这一要求，这要归功于一组专门设计用于在DNA元条形码环境中分析NGS数据的程序。它们在考虑分类注释的同时过滤和编辑序列的能力有助于为广泛的DNA元条形码应用建立量身定制的分析管道，包括生物多样性调查或饮食分析。OBITOOLS软件包是作为一个开源软件分发的，可在以下网站上获得：http://metabarcoding.org/obitools。银河包装器可在GenOuest核心设施工具箱上获得：http://toolshed.genouest.org。
DNA metabarcoding offers new perspectives in biodiversity research. This recently developed approach to ecosystem study relies heavily on the use of next-generation sequencing (NGS) and thus calls upon the ability to deal with huge sequence data sets. The OBITOOLS package satisfies this requirement thanks to a set of programs specifically designed for analysing NGS data in a DNA metabarcoding context. Their capacity to filter and edit sequences while taking into account taxonomic annotation helps to set up tailor-made analysis pipelines for a broad range of DNA metabarcoding applications, including biodiversity surveys or diet analyses. The OBITOOLS package is distributed as an open source software available on the following website: http://metabarcoding.org/obitools. A Galaxy wrapper is available on the GenOuest core facility toolshed: http://toolshed.genouest.org.