Anti-inflammatory effects of melatonin in a rat model of caerulein-induced acute pancreatitis
Anti-inflammatory effects of melatonin in a rat model of caerulein-induced acute pancreatitis
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DOI：
10.1002/cbf.2942
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发表时间：
2013-10-01
影响因子：
3.6
通讯作者：
Pariente, Jose A.
中科院分区：
文献类型：
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作者：
Carrasco, Cristina;Marchena, Ana M.;Pariente, Jose A.
本研究的目的是评价褪黑激素对雨蛙素诱导的急性胰腺炎大鼠模型的保护作用。为了诱导实验性急性胰腺炎，每隔2小时给Wistar大鼠皮下注射4次雨蛙肽（20 μ g/kg体重）。每次注射雨蛙肽前30 min腹腔注射褪黑激素（25 mg/kg体重）。12 h后断头处死大鼠。采集血液和胰腺样本并分别进行血清学和组织病理学研究。脂肪酶，淀粉酶，皮质酮，总抗氧化能力和细胞因子白细胞介素（IL）-1，IL-4和肿瘤坏死因子（TNF）-使用商业试剂盒进行测定。方差分析和Tukey检验（P
The purpose of our study was to evaluate the protective effect of melatonin in a rat model of caerulein-induced acute pancreatitis. For the induction of experimental acute pancreatitis, four subcutaneous injections of caerulein (20 mu gkg(-1) body weight) were given to Wistar rats at 2-h intervals. Melatonin was injected intraperitoneally (25mgkg(-1) body weight) 30min before each caerulein injection. After 12h, rats were sacrificed by decapitation. Blood and pancreas samples were collected and processed for serological and histopathological studies, respectively. Lipase, -amylase, corticosterone, total antioxidant power and cytokines interleukin (IL)-1, IL-4 and tumour necrosis factor (TNF)- were determined using commercial kits. ANOVA and Tukey tests (P