Quantification of laminarialean zoospores in seawater by real‐time PCR
Quantification of laminarialean zoospores in seawater by real‐time PCR
复制标题
通过实时 PCR 定量海水中的海带游动孢子
DOI：
10.1111/pre.12384
复制
发表时间：
2020
影响因子：
1.5
通讯作者：
T. Motomura
中科院分区：
文献类型：
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作者：
Chikako Nagasato;H. Kawamoto;T. Tomioka;S. Tsuyuzaki;Chika Kosugi;Toshiaki Kato;T. Motomura
海带植物在宏观的二倍体孢子体和微观的单倍体配子体之间经历了几代的异型交替。通过形成和释放无性或有性生殖细胞来改变世代。监测游动孢子在环境中的释放和扩散是很困难的。此外，即使可以检测到游动孢子，物种鉴定仍然很困难。本研究试图建立一种快速、灵敏的海带游动孢子定量PCR鉴定方法。此外，我们旨在估计天然海水中游动孢子的密度。设计了甘蔗和裙带菜的特异引物，用于估算海水中游动孢子的数量。在同一海域连续2年，每月采集1 - 2次海水DNA样本（环境DNA），监测游动孢子释放的季节变化。本研究中基于释放游动孢子数量估算的成熟周期与先前报道的日本稻和羽带菜孢子体的成熟周期相当。这支持了我们的方法在估算海带植物游动孢子释放量方面的有效性。该方法将为这些具有商业价值的物种的生态学研究提供有用的工具。
Laminarialean plants undergo heteromorphic alternation of generations between the macroscopic diploid sporophyte and the microscopic haploid gametophyte. The generations change through the formation and release of asexual or sexual reproductive cells. It is difficult to monitor the release and diffusion of zoospores into the environment. Furthermore, even if zoospores can be detected, species identification remains difficult. This study attempted to develop a sensitive and fast identification method for laminarialean zoospores using quantitative PCR. In addition, we aimed to estimate the density of zoospores in natural seawater. Specific primers for Saccharina japonica and Undaria pinnatifida were designed and used to estimate the quantity of zoospores in seawater. DNA samples (environmental DNA) were collected from seawater once or twice each month for 2 years at the same area, and seasonal variations in the release of zoospores was monitored. The estimated maturation period based on the number of released zoospores in this study was comparable with those of the previously reported maturation periods of S. japonica and U. pinnatifida sporophytes. This supports the validity of our method in estimating zoospore release from laminarialean plants. The method will be a useful tool for ecological studies on these commercially relevant species.
1945 年以来的生物恐怖主义
DOI：
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发表时间：
2005
期刊：
Deadly Cultures : Biological weapons since 1945 (Mark Wheelis, Lajos Rozsa & Malcolm Dando) (Harvard University Press) (Forthcoming)
影响因子：
--
作者：
Mark Wheelis;Masaaki Sugishima
通讯作者：
Masaaki Sugishima