Mapping of cerebro-vascular blood perfusion in mice with skin and skull intact by Optical Micro-AngioGraphy at 1.3μm wavelength
Mapping of cerebro-vascular blood perfusion in mice with skin and skull intact by Optical Micro-AngioGraphy at 1.3μm wavelength
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DOI：
10.1364/oe.15.011402
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发表时间：
2007-09-03
期刊：
影响因子：
3.8
通讯作者：
Hurst, Sawan
中科院分区：
文献类型：
--
作者：
Wang, Ruikang K.;Hurst, Sawan
光学微血管造影（OMAG）的发展，以实现体积成像的微结构和动态脑血管血流灌注在小鼠毛细血管水平的分辨率和高信号背景比。在本文中，我们提出了一种高速和高灵敏度的OMAG成像系统，使用InGaAs线扫描相机和宽带光源在1.3 μ m波长的增强组织成像深度。我们表明，高质量的成像脑血管血液灌注下降到毛细血管水平的分辨率与完整的皮肤和颅骨在体内获得与OMAG，没有干扰的血液灌注在覆盖的皮肤。结果表明，1.3 μ m的OMAG在人类和小动物研究中的血液灌注的高速和高灵敏度成像的潜力。(C)2007年，美国光学学会。
Optical micro-angiography (OMAG) was developed to achieve volumetric imaging of the microstructures and dynamic cerebrovascular blood perfusion in mice with capillary level resolution and high signal-to-background ratio. In this paper, we present a high-speed and high-sensitivity OMAG imaging system by using an InGaAs line scan camera and broadband light source at 1.3 mu m wavelength for enhanced imaging depth in tissue. We show that high quality imaging of cerebrovascular blood perfusion down to capillary level resolution with the intact skin and cranium are obtained in vivo with OMAG, without the interference from the blood perfusion in the overlaying skin. The results demonstrate the potential of 1.3 mu m OMAG for high-speed and high-sensitivity imaging of blood perfusion in human and small animal studies. (C) 2007 Optical Society of America.