The specific localization of seminolipid molecular species on mouse testis during testicular maturation revealed by imaging mass spectrometry
The specific localization of seminolipid molecular species on mouse testis during testicular maturation revealed by imaging mass spectrometry
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DOI：
10.1093/glycob/cwp089
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发表时间：
2009-09-01
期刊：
影响因子：
4.3
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Goto-Inoue, Naoko;Hayasaka, Takahiro;Setou, Mitsutoshi
哺乳动物睾丸中90%以上的糖脂由一种独特的硫酸化甘油糖脂组成，称为胆固醇脂。分子的半乳糖基化由UDP-半乳糖：神经酰胺半乳糖基转移酶（CGT）催化。小鼠CGT基因的破坏会导致精子发生停滞而导致男性不育，这表明精脂在生殖功能中发挥着重要作用。基于脂肪酸组成，可以将精脂分子分配到不同的分子种类。在本报告中，我们通过成像质谱法研究了胆固醇脂分子种类的定位，并证明主要分子（C16：0-烷基-C16：0-酰基）在整个小管中表达：一些（C16：0-烷基-C14：0-酰基和C14：0-烷基-C16：0-酰基）主要在精母细胞中表达，另一个（C17：0-烷基-C16：0-酰基）在精子细胞和精子中特异性表达。这是第一份报告，以显示在睾丸成熟过程中的细胞特异性定位的每个分子种类的胆固醇脂。
More than 90% of the glycolipid in mammalian testis consists of a unique sulfated glyceroglycolipid called seminolipid. The galactosylation of the molecule is catalyzed by UDP-galactose:ceramide galactosyltransferase (CGT). Disruption of the CGT gene in mice results in male infertility due to the arrest of spermatogenesis, indicating that seminolipid plays an important role in reproductive function. Seminolipid molecules can be assigned to different molecular species based on the fatty acid composition. In this report, we investigated the localizations of the molecular species of seminolipid by imaging mass spectrometry and demonstrated that major molecule (C16:0-alkyl-C16:0-acyl) was expressed throughout the tubules: some (C16:0-alkyl-C14:0-acyl and C14:0-alkyl-C16:0-acyl) were predominantly expressed in spermatocytes and the other (C17:0-alkyl-C16:0-acyl) was specifically expressed in spermatids and spermatozoa. This is the first report to show the cell-specific localization of each molecular species of seminolipid during testicular maturation.