12 Functions of the hsp70 Protein Family: An Overview
12 Functions of the hsp70 Protein Family: An Overview
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hsp70 蛋白家族的 12 种功能：概述
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
H. Pelham
中科院分区：
文献类型：
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作者：
H. Pelham
自从热休克蛋白被发现以来，人们就一直在猜测它们的功能(Tissieres等人。1974年)。早期的一个假设是，它们以某种方式恢复了经历了急性和潜在破坏性压力的细胞的现状，并保护细胞免受进一步的伤害。1980年，HighTower指出，大多数应激反应的诱导剂都会导致细胞中变性或受损蛋白质的积累，并提出热休克蛋白以某种方式帮助处理这种物质。近年来，这些概括得到了对热休克蛋白功能的更具体研究的支持，这些研究已经导致了至少其中一些作用机制的可信的分子模型。同样清楚的是，热休克蛋白或密切相关的蛋白质存在于未受压力的细胞中，并执行正常生长所必需的功能。在本章中，我讨论了HSP70家族，并认为这些蛋白质(以及其他一些HSPs)的作用是促进因高温或其他滥用而变性的蛋白质的重新折叠和重组。这一作用是HSP70家族在非应激细胞中的一项功能的延伸，即帮助新合成的蛋白质组装。I.热休克蛋白70在应激细胞中的定位热休克蛋白70通常是应激细胞蛋白质合成的最主要产物，而在热休克果蝇细胞中，[35 S]蛋氨酸大部分掺入到这一蛋白质中。因此很容易跟上..。
There has been speculation about the function of heat shock proteins ever since their discovery (Tissieres et al. 1974). An early assumption was that they somehow serve to restore the status quo in cells that have experienced an acute and potentially damaging stress, and to protect against further injury. In 1980, Hightower noted that most inducers of the stress response lead to the accumulation of denatured or damaged proteins in cells and suggested that hsps somehow help to deal with this material. In recent years, these generalizations have been backed up by more specific studies of hsp function that have led to plausible molecular models for the mechanism of action of at least some of them. It has also become clear that hsps or closely related proteins are present in unstressed cells and perform functions essential for normal growth. In this chapter, I discuss the hsp70 family and argue that the role of these proteins (as well as that of some other hsps) is to promote the refolding and reassembly of proteins that have become denatured as a result of hyperthermia or other abuse. This role is an extension of one of the functions of the hsp70 family in unstressed cells, which is to aid the assembly of newly synthesized proteins. I. LOCALIZATION OF HSP70 IN STRESSED CELLS hsp70 is often the most prominent product of protein synthesis in stressed cells, and in heat-shocked Drosophila cells, most incorporation of [ 35 S]methionine is into this protein. It is thus easy to follow...