Genome Editing of C. elegans.

Genome Editing of C. elegans.
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线虫的基因组编辑。

DOI:
10.1007/978-1-4939-7128-2_20
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发表时间:
2017
期刊:
Methods Mol Biol.
影响因子:
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通讯作者:
Takuma Sugi
Takuma Sugi
中科院分区:
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文献类型:
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作者:
孫 ユリ;羽鳥 恵;羽鳥恵;Takuma Sugi

文献摘要

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秀丽隐杆线虫是一种长1 mm的自由生活的线虫,是研究各种生物过程的传统模式动物。使C. elegansa是真核生物遗传学研究的强大模型选择,包括其快速的生命周期,注释良好的基因组,简单的形态学和透明度。最近,基因组编辑技术在C.从而促进其遗传分析。在这里,我介绍一个在C中经常使用的协议。elegansgenome editing.
Caenorhabditis elegans, a 1 mm long free-living nematode, is a traditional model animal for genetic investigations of various biological processes. Characteristic features that makeC. elegansa powerful model of choice for eukaryotic genetic studies include its rapid life cycle, well-annotated genome, simple morphology, and transparency. Recently, genome editing technologies have been increasingly used inC. elegans, thereby facilitating their genetic analyses. Here, I introduce a protocol frequently used inC. elegansgenome editing.