Rotating versus perfusion bioreactor for the culture of engineered vascular constructs based on hyaluronic acid
Rotating versus perfusion bioreactor for the culture of engineered vascular constructs based on hyaluronic acid
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DOI：
10.1002/bit.21828
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发表时间：
2008-08-01
影响因子：
3.8
通讯作者：
Remuzzi, Andrea
中科院分区：
文献类型：
--
作者：
Arrigoni, Chiara;Chitto, Annalisa;Remuzzi, Andrea
血管组织工程需要动态的培养条件。我们比较了两个动态培养系统，灌注和旋转生物反应器，使用管状结构的基础上透明质酸接种猪主动脉平滑肌细胞（SMC），我们最近证明是足够的血管组织的产生的影响。在灌注结构中，机械刺激对细胞有重要影响，增加了细胞增殖形态的发生率，减少了凋亡。然而，细胞外基质沉积，细胞骨架组织和机械性能差。在旋转的结构中，细胞增殖也高于静态对照，细胞凋亡低于静态对照。旋转结构显示最高的极限应力和最低的弹性模量。我们的数据表明，旋转生物反应器是更有效的灌注生物反应器，然后我们建议，这种方法可以被认为是一个有效的替代文献中描述的复杂的生物反应器系统。
It is generally accepted that dynamic culture conditions are required for vascular tissue engineering. We compared the effects of two dynamic culture systems, a perfusion and a rotating bioreactor, using tubular constructs based on hyaluronic acid seeded with porcine aortic smooth muscle cells (SMC), that we recently showed to be adequate for the generation of vascular tissue. In perfused constructs mechanical stimulation importantly affected cell, increased the incidence of cell proliferation morphology and reduced apoptosis. However, extracellular matrix deposition, cytoskeletal organization and mechanical properties were poor. In rotated constructs cell proliferation was also higher and apoptosis lower than in static controls. Rotated constructs showed the highest ultimate stress and the lowest elastic modulus. Our data indicate that the rotating bioreactor is more efficient than the perfusion bioreactor and we then suggest that this method can be considered a valid alternative to complex bioreactor systems described in the literature.