Structure and function of zooplankton-associated bacterial communities in a temperate estuary change more with time than with zooplankton species
Structure and function of zooplankton-associated bacterial communities in a temperate estuary change more with time than with zooplankton species
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DOI：
10.3354/ame01676
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发表时间：
2014-01-01
影响因子：
1.4
通讯作者：
Grossart, Hans-Peter
中科院分区：
文献类型：
--
作者：
Bickel, Samantha L.;Tang, Kam W.;Grossart, Hans-Peter
相对于周围的水，浮游动物以较高的浓度支持着不同的细菌群落，但人们对这些细菌群落的组成和功能如何随着时间的推移而与环境条件有关的变化知之甚少。我们对切萨皮克湾支流约克河中与普通浮游动物群落以及自由生活细菌群落相关的细菌群落进行了为期一年的实地研究。分别用16S rDNA变性梯度凝胶电泳法(DGGE)和Biolog EcoPlates法检测细菌群落的遗传指纹及其碳底物的利用。与浮游动物相关的群落在基因上与自由生活的细菌群落不同，但使用了类似的碳底物阵列。平均而言，与不同浮游动物群落相关的细菌在每个月内的遗传相似性(65.4%)高于同一浮游动物群落在不同月份的遗传相似性(28%~30%)，这表明环境条件在形成浮游动物相关细菌群落方面的重要性。与浮游动物相关的细菌相比，与自由生活的细菌相比，碳底物利用的月度变化变化较小，这表明浮游动物的微生境比周围的水更稳定，在原本不利的水柱条件下支持特定的细菌群。
Zooplankton support distinct bacterial communities in high concentrations relative to the surrounding water, but little is known about how the compositions and functionalities of these bacterial communities change through time in relation to environmental conditions. We conducted a year-long field study of bacterial communities associated with common zooplankton groups as well as free-living bacterial communities in the York River, a tributary of Chesapeake Bay. Bacterial community genetic fingerprints and their carbon substrate usage were examined by denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA and by Biolog EcoPlates, respectively. Zooplankton-associated communities were genetically distinct from free-living bacterial communities but utilized a similar array of carbon substrates. On average, bacteria associated with different zooplankton groups were genetically more similar to each other within each month (65.4% similarity) than to bacterial communities of the same zooplankton group from different months (28 to 30% similarity), which suggests the importance of ambient environmental conditions in shaping resident zooplankton-associated bacterial communities. Monthly changes in carbon substrate utilization were less variable for zooplankton-associated bacteria than for free-living bacteria, suggesting that the zooplankton microhabitat is more stable than the surrounding water and supports specific bacterial groups in the otherwise unfavorable conditions in the water column.