Effects of MicroRNA-143 in the differentiation and proliferation of bovine intramuscular preadipocytes
Effects of MicroRNA-143 in the differentiation and proliferation of bovine intramuscular preadipocytes
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MicroRNA-143对牛肌内前脂肪细胞分化和增殖的影响
DOI：
10.1007/s11033-010-0550-z
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发表时间：
2011-10-01
影响因子：
2.8
通讯作者：
Han, ZhaoYu
中科院分区：
文献类型：
--
作者：
Li, HuiXia;Zhang, Zhen;Han, ZhaoYu
MicroRNAs （miRNAs）是一种短的非编码RNA，通过转录后调控基因表达。miRNA-143已被提出在前脂肪细胞的分化中发挥作用。然而，miRNA-143在哺乳动物肌内脂肪细胞分化中的作用和机制尚不清楚。本研究采用天花板培养法，从荷斯坦牛大理石纹肌组织中培养成纤维细胞样前脂肪细胞。体外研究表明，miRNA-143表达上调后，成纤维细胞样前脂肪细胞可向成熟脂肪细胞分化。此外，转染miRNA-143反义抑制剂可显著抑制成纤维细胞样前脂肪细胞的分化，并表现为脂滴储存减少，CCAAT/增强子结合蛋白-α (C/EBPα)和脂肪酸结合蛋白-4 （FABP-4）等关键脂肪细胞调控基因表达下调。相反，转染miRNA-143抑制剂后，细胞增殖增强。综上所述，我们的研究为牛肌内脂肪分化中内源性miRNA-143的刺激提供了第一个证据，这在一定程度上有助于调节脂肪细胞基因的表达。
MicroRNAs (miRNAs) are short non-coding RNA that post-transcriptionally regulates gene expression. miRNA-143 has been proposed to play a role in the differentiation of preadipocytes. However, effects and mechanism of miRNA-143 in the differentiation of mammals intramuscular adipocytes is unknown. In this study, the fibroblast-like preadipocytes were cultured from the marbling muscle tissue of holstein steers by the ceiling culture method. The in vitro studies showed that the fibroblast-like preadipocytes could differentiated into mature adipocytes with up-regulated expression of miRNA-143. Furthermore, the transfection of the fibroblast-like preadipocytes with miRNA-143 antisense inhibitor induced a significant suppression of differentiation, and indicated by decreased storage of lipid droplets and down-regulated expression of key adipocytes regulatory genes such as CCAAT/enhancer-binding protein-α (C/EBPα) and fatty acid binding proteins-4 (FABP-4). On the contrary, cells proliferation were increased with miRNA-143 inhibitor transfection. Taken together, our study provide the first evidence for stimulation of endogenous miRNA-143 in the differentiation of bovine intramuscular fat, which in part contribute to the regulated expression of adipocyte genes.