pH-dependent multilamellar structures in fetal mouse bone: possible involvement of fatty acids in bone mineralization.
pH-dependent multilamellar structures in fetal mouse bone: possible involvement of fatty acids in bone mineralization.
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DOI：
10.1152/ajpcell.1994.266.3.c590
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发表时间：
1994-03
期刊：
影响因子：
--
通讯作者：
T. Takahashi;S. Ueda;K. Takahashi;R. Scow
中科院分区：
文献类型：
--
作者：
T. Takahashi;S. Ueda;K. Takahashi;R. Scow
胎鼠骨中的pH依赖性多层结构：脂肪酸可能参与骨矿化。Am. J.Physiol.266（Cell Physiol.35）：C590-C600，1994。在培养和未培养的胎鼠顶骨中，用含有单宁酸（TA）的戊二醛溶液固定于pH 7.3，在成骨细胞内外均发现了多层结构（MLS）。在TA存在下固定于pH 6.0的骨和骨细胞培养物中，发现被薄层状结构包围的电子透明区域（直径达1.5微米）代替MLS。大的脂滴被发现，在电子透明的地区和MLS的地方，在pH值为7.4的情况下，TA和脱水的程序，没有提取中性脂质的标本固定。冷冻断裂研究表明，磷脂酰胆碱形成MLS在pH 8.1和pH 6.0，而油酸形成MLS在pH 8.1和脂质滴在pH 6.0。因此，脂肪酸可能形成了骨中发现的pH依赖性MLS。这些数据表明，成骨细胞合成和分泌脂肪酸，作为液滴，进入细胞外空间。在用TA处理的标本中，MLS与钙化类骨质的密切关系表明脂肪酸直接参与骨矿化。
pH-dependent multilamellar structures in fetal mouse bone: possible involvement of fatty acids in bone mineralization. Am. J. Physiol. 266 (Cell Physiol. 35): C590-C600, 1994.--Multilamellar structures (MLS) were found inside and outside osteoblasts in cultured and uncultured fetal mouse parietal bone fixed at pH 7.3 with glutaraldehyde solution containing tannic acid (TA). Electron-lucent areas (up to 1.5 microns in diameter) surrounded by thin lamellar structures were found in place of MLS in bone and bone cell cultures fixed at pH 6.0 in the presence of TA. Large lipid droplets were found, in place of electron-lucent areas and MLS, in specimens fixed at pH 7.4 in the absence of TA and dehydrated with a procedure that did not extract neutral lipid. Freeze-fracture studies showed phosphatidylcholine formed MLS at both pH 8.1 and pH 6.0, whereas oleic acid formed MLS at pH 8.1 and lipid droplets at pH 6.0. Thus fatty acids probably formed the pH-dependent MLS found in bone. The data suggest that osteoblasts synthesize and secrete fatty acids, as droplets, into the extracellular space. The close association of MLS with calcifying osteoid in specimens processed with TA suggests that fatty acids are directly involved in bone mineralization.