Interaction of sodium dodecyl sulfate with the hydrophobic fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate
Interaction of sodium dodecyl sulfate with the hydrophobic fluorescent probe, 2-p-toluidinylnaphthalene-6-sulfonate
复制标题
十二烷基硫酸钠与疏水性荧光探针 2-对甲苯胺基萘-6-磺酸盐的相互作用
DOI：
10.1021/j100585a010
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
A. Lukton
中科院分区：
文献类型：
--
作者：
H. Chiang;A. Lukton
在不同的氯化钠浓度下，十二烷基硫酸钠的临界浓度(CMC)可以随2-对甲苯基萘-6-磺酸盐(TNS)荧光强度的增加而增加。得到了TNS与十二烷基硫酸钠胶束相互作用的热力学参数。结合是放热的，涉及到一个正的熵变。TNS与十二烷基硫酸钠胶束的结合自由能为负值，其主要贡献是热焓为负值。盐(NaC L)通过增加TNS与十二烷基硫酸钠胶束之间的正熵变，增加了TNS与十二烷基硫酸钠胶束的缔合常数。结果表明，TNS与十二烷基硫酸钠胶束的结合力是疏水的。讨论了与蛋白质结合的疏水荧光探针的性质。
The critical concentration (cmc) of sodium dodecyl sulfate at various NaCl concentrations can be followed by the increasing fluorescent intensity of 2-p-toluidinylnaphthalene-6-sulfonate (TNS). The thermodynamic parameters of the interaction of TNS and the SDS micelle have been obtained. Binding is exothermic and involves a positive entropy change. The negative value of enthalpy predominately contributes to the negative free energy of binding between TNS and the SDS micelle. The salt (NaCl) increases the asso-ciation constant between TNS and micelle of SDS by increasing the positiveentropy change. The results suggest that the binding force between TNS and the micelle of SDS is hydrophobic. The nature of hydrophobic fluorescent probe bindingwith proteins is discussed.