Column bioleaching of uranium embedded in granite porphyry by a mesophilic acidophilic consortium.
Column bioleaching of uranium embedded in granite porphyry by a mesophilic acidophilic consortium.
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DOI：
10.1016/j.biortech.2011.01.038
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发表时间：
2011-04
影响因子：
11.4
通讯作者：
G. Qiu;Qian Li;R. Yu;Zhanxue Sun;Yajie Liu;Miao Chen;Huaqun Yin;Yage Zhang;Yili Liang
中科院分区：
文献类型：
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作者：
G. Qiu;Qian Li;R. Yu;Zhanxue Sun;Yajie Liu;Miao Chen;Huaqun Yin;Yage Zhang;Yili Liang
从我国几个铀矿山的酸性矿井水样品中富集了一个嗜中温嗜酸菌群。研究了该菌群在柱浸低品位铀中的浸出性能。研究了几种化学参数对塔式反应器中铀萃取的影响。经过97天的柱浸（包括33天的酸浸预浸和64天的生物浸出），铀的浸出率达到96.82%。反映出间接浸出机理优先于直接浸出机理。利用扩增核糖体DNA限制性酶切分析（Amplified Ribosomal DNA Restriction Analysis）对细菌群落结构进行了分析。结果表明，残液表面微生物的多样性高于残液中。嗜酸氧化亚铁硫杆菌是溶液中的优势种，嗜铁细杆菌是残留表面上的优势种。
A mesophilic acidophilic consortium was enriched from acid mine drainage samples collected from several uranium mines in China. The performance of the consortium in column bioleaching of low-grade uranium embedded in granite porphyry was investigated. The influences of several chemical parameters on uranium extraction in column reactor were also investigated. A uranium recovery of 96.82% was achieved in 97days column leaching process including 33days acid pre-leaching stage and 64days bioleaching stage. It was reflected that indirect leaching mechanism took precedence over direct. Furthermore, the bacterial community structure was analyzed by using Amplified Ribosomal DNA Restriction Analysis. The results showed that microorganisms on the residual surface were more diverse than that in the solution. Acidithiobacillus ferrooxidans was the dominant species in the solution and Leptospirillum ferriphilum on the residual surface.