Expression of the survivin gene in prostate cancer: Correlation with clinicopathological characteristics, proliferative activity and apoptosis
Expression of the survivin gene in prostate cancer: Correlation with clinicopathological characteristics, proliferative activity and apoptosis
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DOI：
10.1097/01.ju.0000120317.88372.03
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发表时间：
2004-05-01
影响因子：
6.6
通讯作者：
Shiina, H
中科院分区：
文献类型：
--
作者：
Kishi, H;Igawa, M;Shiina, H
目的：虽然survivin在前列腺癌中的表达已被报道，但survivin表达的临床意义尚不清楚。为了阐明survivin在前列腺癌中的临床意义，我们检测了前列腺癌组织中survivin mRNA的水平，并将这些水平与患者的临床病理特征和前列腺癌组织内的增殖和凋亡水平等参数进行了关联。材料与方法：从1998年7月至2002年12月间接受根治性前列腺切除术的150例患者的前列腺中获得癌组织和匹配的对照组织。150个样本中，82个前列腺癌样本和80个正常前列腺样本均可提取RNA并得到病理证实。以G3PDH基因为对照，使用survivin基因引物进行聚合酶链反应研究。通过线性回归分析，可计算50例患者术前前列腺特异性抗原倍增时间（PSA-DT）。采用免疫组化染色研究增殖细胞核抗原的表达和单链DNA的表达，分别作为增殖活性指标和凋亡指标。结果：在82例前列腺癌样本中，68例（82.9%）和47例（58.8%）的对照样本显示出可检测到的survivin mRNA水平。在65例前列腺癌及匹配对照中，前列腺癌组织中survivin +/- SE的平均表达水平显著高于对照组织（0.079 +/- 0.017 vs 0.025 +/- 0.005, p = 0.003）。短PSA-DT患者（少于2年）的survivin表达显著高于中度PSA-DT患者(2 - 4年，p
Purpose: Although survivin expression has been reported in prostate cancer, the clinical significance of survivin expression remains unclear. To clarify the clinical significance of survivin in prostate cancer we examined survivin mRNA levels in prostate cancer tissues, and correlated these levels with parameters including the clinicopathological characteristics of patients and proliferation and apoptosis levels within prostate cancer tissues.Materials and Methods: Cancer and matched control tissues were obtained from the prostates of 150 patients who were treated with radical prostatectomy between July 1998 and December 2002. Of the 150 samples, RNA could be extracted and pathological confirmation obtained from 82 prostate cancer and 80 normal prostate samples. Polymerase chain reaction studies were performed using primers for the survivin gene with the G3PDH gene serving as control. Preoperative prostate specific antigen doubling time (PSA-DT) could be calculated for 50 patients by linear regression analysis. Immunohistochemical staining was used to study expression of proliferating cell nuclear antigen as an index of proliferative activity and single stranded DNA as an index of apoptosis.Results: Of 82 prostate cancer samples 68 (82.9%) and 47 (58.8%) of 80 control samples exhibited detectable levels of survivin mRNA. In the 65 cases in which RNA could be extracted from prostate cancer and matched control samples, the mean level of survivin expression +/- SE in prostate cancer was significantly higher than that found in control tissues (0.079 +/- 0.017 vs 0.025 +/- 0.005, p = 0.003). The survivin expression in cancers with a short PSA-DT (less than 2 years) was significantly higher than those with a moderate PSA-DT (2 to 4 years, p