Prolonged intratesticular islet allograft survival is not dependent on local steroidogenesis.
Prolonged intratesticular islet allograft survival is not dependent on local steroidogenesis.
复制标题
睾丸内胰岛同种异体移植物存活时间的延长并不依赖于局部类固醇生成。
DOI：
10.1055/s-2007-1010885
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Whittington,KB
中科院分区：
文献类型：
--
作者：
Selawry,HP;Whittington,KB
控制睾丸内器官移植物特权存活的机制尚不清楚。据推测，睾丸类固醇激素、睾酮和/或孕酮水平升高可能是抑制局部免疫反应的原因。本研究的目的是检查大鼠睾丸内胰岛移植物的存活率，其中生殖细胞和Leydig细胞功能已被选择性破坏。通过长期给予GnRH类似物亮丙瑞林，在糖尿病Sprague-Dawley大鼠中诱导化学去势。此外，通过手术切除糖尿病大鼠的垂体，生殖细胞功能和类固醇生成均严重受损。从Wistar-Lewis大鼠中分离胰岛，然后将其植入亮丙瑞林给药大鼠和垂体切除大鼠的腹部睾丸中。对移植大鼠不给予免疫抑制。结果表明，长期移植存活发生在腹部睾丸的生殖细胞，睾酮和孕酮的剥夺。
The mechanisms that control the privileged survival of intratesticular organ allografts are not known. It had been postulated that the elevated levels of testicular steroid hormones, testosterone and/or progesterone, could be responsible for the inhibition of the local immune response. The goals of this study were to examine intratesticular islet allograft survival in rats in which both germ cell and Leydig cell function had been selectively destroyed. A chemical castration was induced in diabetic Sprague-Dawley rats with the chronic administration of a GnRH analog, leuprolide. In addition, both germ cell function and Steroidogenesis were severely impaired by means of the surgical removal of the pituitary in diabetic rats. Pancreatic islets were isolated from Wistar-Lewis rats and were then implanted into the abdominal testes of leuprolide-treated and of hypophysectomized rats. No immunosuppression was given to the grafted rats. The results showed that long-term allograft survival occurred in the abdominal testes deprived of germ cells, of testosterone and of progesterone.