Identification of CXCR4 and CXCL10 as Potential Predictive Biomarkers in Triple Negative Breast Cancer (TNBC)
Identification of CXCR4 and CXCL10 as Potential Predictive Biomarkers in Triple Negative Breast Cancer (TNBC)
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DOI：
10.12659/msm.918281
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发表时间：
2020-01-11
影响因子：
3.1
通讯作者：
Yi, Cui
中科院分区：
文献类型：
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作者：
Chuan, Tian;Li, Tian;Yi, Cui
背景：三阴性乳腺癌（TNBC）的有效治疗仍然是一个深刻的临床挑战，但关键的驱动基因和复杂的信号通路仍然未知。材料/方法：从GSE53752、GSE45827、GSE65194、GSE38959 4个表达谱中采集223例样本（163例TNBC和60例健康乳腺组织），通过R软件进行深度整合分析。我们检测了差异表达基因（DEGs），并筛选了关键基因和富集途径。通过STRING Version: 11.0数据库和Cytoscape软件构建degs相关蛋白-蛋白相互作用（protein-protein interaction， PPI）网络，筛选中心基因。然后，我们通过Oncomine数据库验证拥抱基因表达水平。此外，我们使用Kaplan-Meier绘图仪数据库分析了TNBC患者中心基因的预后价值。结果：在我们的研究中，我们筛选出365个基因，其中包括212个上调基因和153个下调基因。然后，通过12种算法的交叉选出10个枢纽基因。同时，我们通过Kaplan-Meier绘图仪数据库发现，CXCR4和CXCL10过表达是TNBC无复发生存的有利预后因素。结论：我们的研究发现，CXCR4和CXCL10过表达是TNBC患者预后的有利因素。CXCR4和CXCL10可能是TNBC治疗的有效靶点。
Background: Efficacious therapy for triple negative breast cancer (TNBC) continues to be a profound clinical challenge, but the key driven genes and convoluted signaling pathways are still unknown.Material/Methods: A total of 223 samples (163 TNBC and 60 healthy breast tissues) were taken and deeply integrated analyzed by R software from 4 expression profiles in the study, including GSE53752, GSE45827, GSE65194, and GSE38959. We examined differentially expressed genes (DEGs) and screen for critical genes and pathways enrichment. The protein-protein interaction (PPI) network of DEGs-associated was built through the STRING Version: 11.0 database and Cytoscape software to filter the hub gene. Then, we verified hug gene expression levels through the Oncomine database. Also, we analyzed the prognostic value of TNBC patient's hub genes using the Kaplan-Meier plotter database.Results: In our study, we filter out 365 DEGs, including 212 upregulated genes and 153 downregulated genes. Then, 10 hub genes were picked out by the intersection of 12 algorithms. At the same time, we discovered that CXCR4 and CXCL10 overexpression are favorable prognostic factors for recurrence-free survival of TNBC through the Kaplan-Meier plotter database.Conclusions: Our research found that CXCR4 and CXCL10 overexpressed, and they were a favorable prognostic factor in patients with TNBC. CXCR4 and CXCL10 might be effective targets for TNBC therapy.