Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes
Molecular conservation and differential mutation on ORF3a gene in Indian SARS-CoV2 genomes
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DOI：
10.1016/j.ygeno.2020.06.016
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发表时间：
2020-09-01
期刊：
影响因子：
4.4
通讯作者：
Jana, Siddhartha Sankar
中科院分区：
文献类型：
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作者：
Hassan, Sk. Sarif;Choudhury, Pabitra Pal;Jana, Siddhartha Sankar
由于COVID-19大流行导致的全球紧急情况需要对SARS-CoV 2的基因和基因组进行各种研究。在其他重要的蛋白质中，辅助蛋白的作用在宿主中的冠状病毒的复制、感染的调节中是极其重要的。ORF 3a是SARS-CoV 2基因组中最大的辅助蛋白，它调节宿主对病毒感染的反应，因此在发病机制中起重要作用。在这项研究中，试图破译32个印度患者基因组中ORF 3a基因中核苷酸、二聚体、密码子和氨基酸的保守性。印度SARS-CoV 2基因组中ORF 3a基因存在单点和双点突变，提示印度患者SARS-CoV 2毒力特性发生改变。通过基于核苷酸保守性等的系统发育分析，我们发现SARS-CoV 2和Pangolin-CoV基因组中ORF 3a基因的双亲起源是相同的。这项研究强调了印度SARS-CoV 2基因组中ORF 3a突变的积累，这可能会提供针对SARS-CoV 2的设计治疗方法。
A global emergency due to the COVID-19 pandemic demands various studies related to genes and genomes of the SARS-CoV2. Among other important proteins, the role of accessory proteins are of immense importance in replication, regulation of infections of the coronavirus in the hosts. The largest accessory protein in the SARS-CoV2 genome is ORF3a which modulates the host response to the virus infection and consequently it plays an important role in pathogenesis. In this study, an attempt is made to decipher the conservation of nucleotides, dimers, codons and amino acids in the ORF3a genes across thirty-two genomes of Indian patients. ORF3a gene possesses single and double point mutations in Indian SARS-CoV2 genomes suggesting the change of SARS-CoV2's virulence property in Indian patients. We find that the parental origin of the ORF3a gene over the genomes of SARS-CoV2 and Pangolin-CoV is same from the phylogenetic analysis based on conservation of nucleotides and so on. This study highlights the accumulation of mutation on ORF3a in Indian SARS-CoV2 genomes which may provide the designing therapeutic approach against SARS-CoV2.