Geometry of normal mammalian platelets by quantitative microscopic studies.
Geometry of normal mammalian platelets by quantitative microscopic studies.
复制标题
通过定量显微镜研究正常哺乳动物血小板的几何形状。
DOI：
10.1016/s0006-3495(76)85756-6
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发表时间：
1976
影响因子：
3.4
通讯作者：
R. Panjwani
中科院分区：
文献类型：
--
作者：
M. Frojmovic;R. Panjwani
正常和硬化的人和兔红细胞和血小板的形状分布是通过对数百个自由旋转细胞的边缘方向的分析获得的，该显微照片类似于 Ponder (1930，Q.J.Exp.Physiol.20:29)通过相差显微镜在 800 X 放大倍率下获得的显微照片。估计正常细胞和硬化细胞的大平均直径（d）和厚度（t），并用于计算平均几何轴比rp=t/d，随着细胞变得更加球形，该比值增加到统一。我们的固定程序没有改变这些形状参数：红细胞的 rp 没有变化，d 和 t 值与 Ponder (1930) 报道的相似；对于人和兔细胞，血小板分别具有d X t=3.6 +/- 0.7 mum X 0.9 +/- 0.3 mum和3.1 +/- 0.4 mum X 0.6 +/- 0.3 mum，rp分别=0.26和0.20。 rp 与通过新型流变光学方法获得的数据一致，该方法允许对大量群体（大于 100 X 10(3) 细胞）进行直接统计平均。对上述三个参数（d，t，rp）以及为“长椭球体”和“圆边圆盘”模型计算的体积（V）、总表面积（S）和球形指数（S.I.）进行了直方图和线性相关研究。结果表明 rp - t、rp - S.I. 和 d -S 之间具有非常高的线性相关性，t - V、d -V 和 S 之间具有很高的相关性。数据与文献中通过其他方法确定的少数报告一致，血小板的最佳模型似乎是扁球体。
The shape distributions of normal and hardened human and rabbit erythrocytes and platelets were obtained for edge-on orientations of a few hundred freely rotating cells from analyses of microphotographs obtained similarly as by Ponder(1930, Q. J. Exp. Physiol. 20:29) by phase-contrast microscopy at 800 X magnification. Major average diameters (d) and thicknesses (t) were estimated for both normal and hardened cells, and were used to calculate an average geometric axis ratio, rp=t/d, which increases to unity as cells become more spherical. Our fixation procedure did not alter these shape parameters: rp was unchanged for erythrocytes, with d and t values similar to those reported by Ponder (1930); platelets had d X t=3.6 +/- 0.7 mum X 0.9 +/- 0.3 mum and 3.1 +/- 0.4 mum X 0.6 +/- 0.3 mum, respectively, for human and rabbit cells, with rp=0.26 and 0.20, respectively. Agreement in rp was found with data obtained by a novel rheo-optical method which allows for a direct statistical averaging for large populations (greater than 100 X 10(3) cells). Histograms and linear correlation studies were made of the above three parameters (d,t,rp), as well as volume (V), total surface area are (S), and sphericity index (S.I.) calculated for both "prolate ellipsoid" and "disc with rounded edges" models. Results indicate very high linear correlations between rp - t, rp - S. I., and d -S, with high correlations for t - V,d -V and S. Data are in agreement with the few reports in the literature determined by other methods, with the best model for platelets appearing to be an oblate spheroid.