A rare-cell detector for cancer
A rare-cell detector for cancer
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DOI：
10.1073/pnas.0404036101
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发表时间：
2004-07-20
影响因子：
11.1
通讯作者：
Bruce, RH
中科院分区：
文献类型：
--
作者：
Krivacic, RT;Ladanyi, A;Bruce, RH
尽管可靠的血液癌细胞检测方法将是早期诊断和监测实体瘤的重要工具，但目前的技术无法可靠地检测这些极低浓度的罕见细胞。首选的检测方法，自动数字显微镜（ADM），是太慢，扫描大基板面积。在这里，我们报告的方法，使用光纤阵列扫描技术（FAST），它适用于激光打印技术的稀有细胞检测问题。使用FAST细胞仪，激光打印光学器件每秒可激发300，000个细胞，并在极宽的视野内收集发射，使速度比ADM快500倍，具有相当的灵敏度和上级特异性。FAST富集和ADM成像的组合具有可靠检测血液中早期癌症所需的性能。
Although a reliable method for detection of cancer cells in blood would be an important tool for diagnosis and monitoring of solid tumors in early stages, current technologies cannot reliably detect the extremely low concentrations of these rare cells. The preferred method of detection, automated digital microscopy (ADM), is too slow to scan the large substrate areas. Here we report an approach that uses fiber-optic array scanning technology (FAST), which applies laser-printing techniques to the rare-cell detection problem. With FAST cytometry, laser-printing optics are used to excite 300,000 cells per sec, and emission is collected in an extremely wide field of view, enabling a 500-fold speed-up over ADM with comparable sensitivity and superior specificity. The combination of FAST enrichment and ADM imaging has the performance required for reliable detection of early-stage cancer in blood.