SPONTANEOUS DEGENERATION OF GERM-CELLS IN NORMAL RAT TESTIS - ASSESSMENT OF CELL-TYPES AND FREQUENCY DURING THE SPERMATOGENIC CYCLE
SPONTANEOUS DEGENERATION OF GERM-CELLS IN NORMAL RAT TESTIS - ASSESSMENT OF CELL-TYPES AND FREQUENCY DURING THE SPERMATOGENIC CYCLE
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DOI：
10.1530/jrf.0.0950825
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发表时间：
1992-08-01
期刊：
影响因子：
--
通讯作者：
KERR, JB
中科院分区：
文献类型：
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作者：
KERR, JB
在8只正常成年大鼠的睾丸组织中测量了生精上皮周期中退化生殖细胞的发生。睾丸灌注固定，包埋在环氧树脂中，切片后，共180个随机选择的块在1 μ m，染色切片进行了检查，通过光学显微镜，所有横截面的曲细精管被归类为14个阶段的生精周期之一。在2103个小管中记录每个小管的变性细胞数。退化的生殖细胞没有检测到在阶段II-VI，只有很少在阶段VII（n = 366小管），其中一个初级精母细胞和一个步骤19精子细胞退化。所有其他阶段表现出更大的生殖细胞变性的发生率，特别是在第XIV阶段，平均而言，每个圆形生精小管的变性细胞的频率是第VII阶段的约40倍。结果表明，在正常成年大鼠睾丸中，生殖细胞在经历VII期时退化的风险最小。
The occurrence of degenerating germ cells in the cycle of the seminiferous epithelium was measured in testicular tissues from eight normal adult rats. Testes were perfusion fixed, embedded in epoxy resin and, after sectioning a total of 180 randomly selected blocks at 1-mu-m, stained sections were examined by light microscopy; all cross-sectioned seminiferous tubules were categorized into one of 14 stages of the spermatogenic cycle. The number of degenerating cells per tubule was recorded in 2103 tubules. Degenerating germ cells were not detected at stages II-VI, and only rarely at stage VII (n = 366 tubules) in which one primary spermatocyte and one step 19 spermatid degenerated. All other stages exhibited a greater incidence of degenerative germ cells, particularly at stage XIV where, on average, the frequency of degenerating cells per round seminiferous tubule was about 40 times greater than at stage VII. The results indicated that, in the normal adult rat testis, the germ cells are least at risk of degeneration as they pass through stage VII.