Dietary Taurine Attenuates Dextran Sulfate Sodium (DSS)-induced Experimental Colitis in Mice
Dietary Taurine Attenuates Dextran Sulfate Sodium (DSS)-induced Experimental Colitis in Mice
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DOI：
10.1007/978-0-387-75681-3_27
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Satsu, Hideo
中科院分区：
文献类型：
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作者：
Shimizu, Makoto;Zhao, Zhaohui;Satsu, Hideo
研究了牛磺酸对葡聚糖硫酸钠（DSS）诱导的实验性结肠炎的影响。C57 BL/6小鼠连续5天给予牛磺酸或安慰剂，以3%DSS诱导急性。采用腹泻/出血评分、结肠长度变化、组织学评分和组织髓过氧化物酶（MPO）活性等指标对结肠炎进行评估。此外，通过实时PCR测定白细胞介素（IL）-1 β、肿瘤坏死因子（TNF）-α和巨噬细胞炎性蛋白（MIP）-2的组织mRNA水平。补充牛磺酸可显著减轻腹泻、结肠缩短、组织学评分、MPO活性升高和MIP-2基因表达异常的严重程度，表明牛磺酸可预防DSS诱导的结肠炎。牛磺酸还抑制TNF-α诱导的人肠上皮Caco-2细胞分泌IL-8（MIP-2的人同源物）。抑制肠细胞分泌趋化因子可能与牛磺酸对肠上皮细胞的保护作用有关。
Effects of dietary taurine on the experimental colitis induced by dextran sulfate sodium (DSS) were studied. C57BL/6 mice administrated taurine or placebo for 5 days were given 3% DSS to induce acute. The colitis was as-sessed using indices such as diarrhea/bleeding scores, colon length change, histological score and tissue myeloperoxidase (MPO) activity. Further, tis-sue mRNA levels of interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha and macrophage inflammatory protein (MIP)-2, were determined by real-time PCR. Taurine supplementation significantly attenuated the severity of diarrhea, colon shortening, histological score, MPO activity elevation and abnormal MIP-2 gene expression, indicating that taurine prevents DSS-induced colitis. Taurine also inhibited the TNF-alpha-induced secretion of IL-8 (a hu-man homologue of MIP-2) from human intestinal epithelial Caco-2 cells. Inhibition of chemokine secretion from intestinal cells may be involved in the mechanisms underlying the cytoprotective function of taurine in the intestinal epithelium.