PHOTOTHERMAL DEFLECTION SPECTROSCOPY OF PHOTOSYNTHETIC PIGMENTS in vivo and in vitro
PHOTOTHERMAL DEFLECTION SPECTROSCOPY OF PHOTOSYNTHETIC PIGMENTS in vivo and in vitro
复制标题
光合成色素体内外光热偏转光谱
DOI：
10.1111/j.1751-1097.1990.tb01743.x
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发表时间：
1990
影响因子：
3.3
通讯作者：
R. Leblanc
中科院分区：
文献类型：
--
作者：
L. Lorrain;M. Havaux;A. Tessier;R. Leblanc
摘要-本技术说明描述了光热光束偏转装置的设计，该装置可以方便快速地测量各种光合材料（包括整株植物叶片）中吸收光能的热耗散。该系统基于“海市蜃楼效应”，其中在与样品接触的流体中诱导的折射率梯度，用强度调制光照射，导致平行于样品表面的激光束的周期性偏转。探测激光束的偏转由位置传感器检测，其输出由锁定放大器处理。光热偏转信号可以在各种（液体或气体）环境中放置的完整叶片中进行体内监测，在低调制频率（约30 Hz）下具有100（水中）和50（空气中）之间的令人满意的信噪比。它表明，这种新的和简单的光热技术是一个非常敏感的工具，用于测量吸收光谱的光合色素在体内（叶，藻类或叶绿体）和模型系统（朗缪尔-布洛杰特和固体膜的叶绿素）。
Abstract— This Technical Note describes the design of a photothermal beam deflection apparatus which allows the easy and rapid measurement of thermal dissipation of absorbed light energy in various photosynthetic materials including whole plant leaves. This system is based on the “mirage effect” in which the refractive index gradient induced in a fluid in contact with the sample, irradiated with an intensity‐modulated light, causes the periodic deflection of a laser beam parallel to the sample surface. The deflection of the probe laser beam is detected by a position sensor, the output of which is processed by a lock‐in amplifier. Photothermal deflection signals can be monitored in vivo in intact leaves placed in various (liquid or gaseous) environments with a satisfactory signal‐to‐noise ratio between 100 (in water) and 50 (in air) at low modulation frequencies (ca 30 Hz). It is shown that this new and simple photothermal technique is a very sensitive tool for the measurement of absorption spectra of photosynthetic pigments both in vivo (leaves, algae or chloroplasts) and in model systems (Langmuir‐Blodgett and solid films of chlorophyll).