CSF proteome analysis in multiple sclerosis patients by two-dimensional electrophoresis
CSF proteome analysis in multiple sclerosis patients by two-dimensional electrophoresis
复制标题
DOI：
10.1111/j.1468-1331.2008.02239.x
复制
发表时间：
2008-09-01
影响因子：
5.1
通讯作者：
Sarchielli, P.
中科院分区：
文献类型：
--
作者：
Chiasserini, D.;Di Filippo, M.;Sarchielli, P.
近年来，不同的方法已被用于研究受多发性硬化（MS）影响的患者的脑脊液（CSF）蛋白质组的变化，旨在确定具有潜在诊断或预后价值的蛋白质标志物。由于目前的蛋白质组学技术缺乏标准化，到目前为止，在不同的实验室取得了对比结果。在这项研究中，我们比较了10例复发-缓解型MS（RR-MS）患者，11例临床孤立综合征（CIS）患者，和10名无神经系统或全身性疾病的对照受试者。我们发现CIS和RR-MS患者中IgG游离κ轻链蛋白的过表达，与对照组相比，RR-MS患者中载脂蛋白E亚型的表达增加。我们的研究结果证实了MS患者CSF蛋白质组的变化。未来的研究应旨在研究这些候选CSF标志物在更大的CIS和MS患者队列中的作用。
In recent years, different approaches have been used to investigate changes of cerebrospinal fluid (CSF) proteome in patients affected by multiple sclerosis (MS) with the aim to identify protein markers with potential diagnostic or prognostic value. Because of the lack of standardization of current proteomic techniques, contrasting results were achieved until now in different laboratories. In this study, we compare CSF proteome of 10 relapsing-remitting MS (RR-MS) patients, 11 patients with clinically isolated syndrome (CIS), and 10 control subjects without neurological or systemic diseases.The differential expression of CSF proteins amongst these cohorts of patients was investigated by using two-dimensional electrophoresis and mass spectrometry.We found an overexpression of IgG free kappa light chain protein in both CIS and RR-MS patients, compared with control subjects and an increased expression of an apolipoprotein E isoform in RR-MS patients, compared with CIS and control groups. Our results confirm the presence of CSF proteome changes in MS patients. Future research should be aimed to investigate the role of these candidate CSF markers in larger cohorts of CIS and MS patients.