Plasma Oxylipins: A Potential Risk Assessment Tool in Atherosclerotic Coronary Artery Disease.
Plasma Oxylipins: A Potential Risk Assessment Tool in Atherosclerotic Coronary Artery Disease.
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DOI：
10.3389/fcvm.2021.645786
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发表时间：
2021
影响因子：
3.6
通讯作者：
Kaul S
中科院分区：
文献类型：
--
作者：
Le DE;García-Jaramillo M;Bobe G;Alcazar Magana A;Vaswani A;Minnier J;Jump DB;Rinkevich D;Alkayed NJ;Maier CS;Kaul S
背景：虽然氧脂素与冠状动脉疾病（CAD）有关，但对其诊断和预后潜力知之甚少。目的：我们测试了特定氧脂素的血浆浓度是否可以区分患病冠状动脉的数量，并预测有症状成人的中位5年结局。研究方法：采用高效液相色谱（HPLC）和定量串联质谱法相结合，我们对来自俄勒冈州大波特兰地区的23名无症状低CAD风险成人和74名有症状成人（≥70%狭窄）的血浆样本中的39种氧脂素进行了有针对性的分析。在>98%的成年人中，22种氧脂素的浓度高于定量下限，并根据前体和生物合成途径，在有症状的成年人中单独和分组比较冠状动脉病变数量[（1）n = 31;（2）n = 23;（3）n = 20]和中位5年随访期间的结果（无手术：n = 7;冠状动脉支架置入术：n = 24;冠状动脉旁路移植术：n = 26;死亡：n = 7）。结果如下：六个量化的氧脂素的血浆水平随着患病动脉的数量而降低;一组五个氧脂素诊断出三个患病动脉，灵敏度为100%，特异性为70%。五种氧脂素的浓度较低，一种氧脂素的浓度较高;一组两种氧脂素预测随访期间的生存率，敏感性为86%，特异性为91%。结论：结合标准风险评估工具，血浆氧化脂质定量可能有助于CAD诊断和预后。
Background: While oxylipins have been linked to coronary artery disease (CAD), little is known about their diagnostic and prognostic potential. Objective: We tested whether plasma concentration of specific oxylipins may discriminate among number of diseased coronary arteries and predict median 5-year outcomes in symptomatic adults. Methods: Using a combination of high-performance liquid chromatography (HPLC) and quantitative tandem mass spectrometry, we conducted a targeted analysis of 39 oxylipins in plasma samples of 23 asymptomatic adults with low CAD risk and 74 symptomatic adults (≥70% stenosis), aged 38–87 from the Greater Portland, Oregon area. Concentrations of 22 oxylipins were above the lower limit of quantification in >98% of adults and were compared, individually and in groups based on precursors and biosynthetic pathways, in symptomatic adults to number of diseased coronary arteries [(1) n = 31; (2) n = 23; (3) n = 20], and outcomes during a median 5-year follow-up (no surgery: n = 7; coronary stent placement: n = 24; coronary artery bypass graft surgery: n = 26; death: n = 7). Results: Plasma levels of six quantified oxylipins decreased with the number of diseased arteries; a panel of five oxylipins diagnosed three diseased arteries with 100% sensitivity and 70% specificity. Concentrations of five oxylipins were lower and one oxylipin was higher with survival; a panel of two oxylipins predicted survival during follow-up with 86% sensitivity and 91% specificity. Conclusions: Quantification of plasma oxylipins may assist in CAD diagnosis and prognosis in combination with standard risk assessment tools.