Spiculogenesis in the sea urchin embryo: Studies on the SM30 spicule matrix protein
Spiculogenesis in the sea urchin embryo: Studies on the SM30 spicule matrix protein
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海胆胚胎中的针状发生：SM30 针状基质蛋白的研究
DOI：
10.1046/j.1440-169x.1995.00008.x
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发表时间：
1995
期刊：
影响因子：
4.6
通讯作者：
W. Lennarz
中科院分区：
文献类型：
--
作者：
Martin F. Brown;J. Partin;C. Killian;W. Lennarz
当分离的蛋白质从刺的圆海胆purpuratus胚胎进行了检查，通过蛋白质印迹分析，观察到约43 kDa的主要蛋白质与单克隆抗体，mAb 1223反应。先前的研究已经确定，该抗体识别细胞表面糖蛋白msp130上的天冬酰胺连接的阴离子碳水化合物表位。这种蛋白质已被证明是特别与主要间充质细胞参与组装的骨针。此外，一些证据表明，碳水化合物表位在Ca 2+沉积到生长的骨针。用mAb 1223检测的43 kDa骨针基质蛋白也与已知骨针基质蛋白SM 30的多克隆抗体反应。进一步的表征实验，包括使用PNGaseF的去糖基化、二维电泳和免疫沉淀，证实了43 kDa针状基质蛋白的pI约为4.0，含有单克隆抗体mAb 1223识别的碳水化合物表位，并与抗SM 30反应。电子显微镜证实脱矿针状体中存在与mAb 1223和抗SM 30反应的蛋白质。我们的结论是，骨针基质蛋白，SM30，是一种糖蛋白，含有糖链相似或相同的主要间充质细胞膜糖蛋白，MSP 130。
When proteins isolated from spicules of Strongylocentrotus purpuratus embryos were examined by western blot analysis, a major protein of approximately 43 kDa was observed to react with the monoclonal antibody, mAb 1223. Previous studies have established that this antibody recognizes an asparagine‐linked, anionic carbohydrate epitope on the cell surface glycoprotein, msp130. This protein has been shown to be specifically associated with the primary mesenchyme cells involved in assembly of the spicule. Moreover, several lines of evidence have implicated the carbohydrate epitope in Ca2+ deposition into the growing spicule. The 43 kDa, spicule matrix protein detected with mAb 1223 also reacted with a polyclonal antibody to a known spicule matrix protein, SM30. Further characterization experiments, including deglycosylation using PNGaseF, two‐dimensional electrophoresis, and immunoprecipitation, verified that the 43 kDa spicule matrix protein had a pl of approximately 4.0, contained the carbohydrate epitope recognized by monoclonal antibody mAb 1223 and reacted with anti‐SM30. Electron microscopy confirmed the presence of proteins within the demineralized spicule that reacted with mAb 1223 and anti‐SM30. We conclude that the spicule matrix protein, SM30, is a glycoprotein containing carbohydrate chains similar or identical to those on the primary mesenchyme cell membrane glycoprotein, msp130.
参与海胆胚胎骨架形成的三种初级间充质细胞特异性糖蛋白常见的翻译后修饰的表征。
影响因子：
2.7
作者：
Kabakoff,B;Hwang,SP;Lennarz,WJ
通讯作者：
Lennarz,WJ
海胆胚胎中初级间充质谱系特异性细胞表面蛋白 msp130 的定位和表达。
DOI：
10.1242/dev.101.2.255
发表时间：
1987
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Anstrom,JA;Chin,JE;Leaf,DS;Parks,AL;Raff,RA
通讯作者：
Raff,RA