Nano-Graphene Oxide for Cellular Imaging and Drug Delivery
Nano-Graphene Oxide for Cellular Imaging and Drug Delivery
复制标题
DOI：
10.1007/s12274-008-8021-8
复制
发表时间：
2008-09-01
期刊：
影响因子：
9.9
通讯作者：
Dai, Hongjie
中科院分区：
文献类型：
--
作者：
Sun, Xiaoming;Liu, Zhuang;Dai, Hongjie
二维石墨烯提供了有趣的电子，热和机械性能，目前正在探索先进的电子，膜和复合材料。在这里，我们合成和探索纳米氧化石墨烯（NGO）的生物应用，即，单层氧化石墨烯片的横向宽度低至几纳米。我们开发了功能化化学，以赋予NGO在生物环境中的溶解性和相容性。我们获得尺寸分离的聚乙二醇化NGO片，其可溶于缓冲液和血清中而不附聚。非政府组织片被发现是在可见光和红外区域的光致发光。非政府组织的固有光致发光（PL）用于活细胞成像在近红外（NIR），几乎没有背景。我们发现，通过π-堆积的简单物理吸附可用于将阿霉素（一种广泛使用的癌症药物）负载到用抗体官能化的NGO上，用于体外选择性杀死癌细胞。由于其尺寸小、固有的光学性质、大的比表面积、低的成本以及与芳香族药物分子的有用的非共价相互作用，NGO是一种有前途的生物和医学应用的新材料。
Two-dimensional graphene offers interesting electronic, thermal, and mechanical properties that are currently being explored for advanced electronics, membranes, and composites. Here we synthesize and explore the biological applications of nano-graphene oxide (NGO), i.e., single-layer graphene oxide sheets down to a few nanometers in lateral width. We develop functionalization chemistry in order to impart solubility and compatibility of NGO in biological environments. We obtain size separated pegylated NGO sheets that are soluble in buffers and serum without agglomeration. The NGO sheets are found to be photoluminescent in the visible and infrared regions. The intrinsic photoluminescence (PL) of NGO is used for live cell imaging in the near-infrared (NIR) with little background. We found that simple physisorption via pi-stacking can be used for loading doxorubicin, a widely used cancer drug onto NGO functionalized with antibody for selective killing of cancer cells in vitro. Owing to its small size, intrinsic optical properties, large specific surface area, low cost, and useful non-covalent interactions with aromatic drug molecules, NGO is a promising new material for biological and medical applications.