In vivo dynamics of anti-viral CD8 T cell responses to different epitopes. An evaluation of bystander activation in primary and secondary responses to viral infection.
In vivo dynamics of anti-viral CD8 T cell responses to different epitopes. An evaluation of bystander activation in primary and secondary responses to viral infection.
复制标题
抗病毒 CD8 T 细胞对不同表位反应的体内动态。
DOI：
10.1007/978-1-4615-5355-7_14
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发表时间：
1998
影响因子：
--
通讯作者：
Ahmed,R
中科院分区：
文献类型：
--
作者：
Murali-Krishna,K;Altman,JD;Suresh,M;Sourdive,D;Zajac,A;Ahmed,R
病毒感染可诱导体内大量T细胞增殖，但只有一小部分（1-5%）的活化T细胞是病毒特异性的，这就导致人们普遍认为大多数T细胞的增殖是病毒介导的旁观者活化和/或非特异性细胞的交叉反应性刺激。为了重新检查这个问题，我们使用三种敏感技术在小鼠的急性LCMV感染期间定量抗原特异性CD 8 T细胞：（i）细胞内细胞因子产生，（ii）单细胞ELISPOT和（iii）通过用MHC I类四聚体+肽染色直接可视化抗原特异性CD 8 T细胞。与先前的估计相反，我们发现50-70%的活化的CD 8 T细胞是LCMV特异性的。这表示在8天内增加≥ 10，000倍（约2 × 107个病毒特异性细胞/脾），在第3天和第5天之间出现扩增峰值，在此期间，病毒特异性CD 8 T细胞的估计分裂时间约为8小时。病毒清除后，抗原特异性CD 8 T细胞的数量降至1 × 106/脾，并在小鼠的一生中维持在该水平。在用LCMV再激发后，记忆性CD 8 T细胞迅速增殖并再次占总CD 8 T细胞的>50%。相反，在用异源病毒如牛痘攻击时，LCMV特异性记忆CTL的数量没有变化，尽管活化的CD 8 T细胞的数量显著增加。总之，这些结果表明，在病毒感染期间观察到的大部分CD 8 T细胞扩增代表抗原特异性细胞。
Viral infections induce extensive T cell proliferationin vivo.However, only a small fraction (1–5%) of the activated T cells have been shown to be virus specific leading to the prevailing notion that most of the T cell expansion represents cytokine-mediated bystander activation and/or cross reactive stimulation of non specific cells. To re-examine this issue we quantitated antigen specific CD8 T cells during acute LCMV infection of mice using three sensitive techniques: (i) intracellular cytokine production, (ii) single cell ELISPOT and (iii) direct visualization of antigen specific CD8 T cells by staining with MHC class I tetramers + peptide. In contrast to previous estimates, we found that 50–70% of the activated CD8 T cells were LCMV specific. This represented ≥ 10,000-fold increase (~2 × 107virus specific cells/spleen) in 8 days with the peak expansion occurring between day 3 and 5 during which period virus specific CD8 T cells had an estimated division time of ~8 hours. Following viral clearance, the number of antigen specific CD8 T cells dropped to 1 × 106per spleen and were maintained at this level for the life of the mouse. Upon rechallenge with LCMV, memory CD8 T cells rapidly proliferated and again comprised >50% of the total CD8 T cells. In contrast, upon challenge with a heterologous virus such as vaccinia, there was no change in the number of LCMV specific memory CTL, despite a substantial increase in the number of activated CD8 T cells. Taken together, these results show that much of the CD8 T cell expansion seen during viral infection represents antigen specific cells.
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影响因子：
2.2
作者：
T. Taguchi;J. Mcghee;R. Coffman;K. Beagley;J. Eldridge;K. Takatsu;H. Kiyono
通讯作者：
T. Taguchi;J. Mcghee;R. Coffman;K. Beagley;J. Eldridge;K. Takatsu;H. Kiyono
影响因子：
4.4
作者：
R. V. D. Most;A. Sette;C. Oseroff;J. Alexander;K. Murali-Krishna;Lisa. L. Lau;S. Southwood;J. Sidney;R. Chesnut;M. Matloubian;R. Ahmed
通讯作者：
R. V. D. Most;A. Sette;C. Oseroff;J. Alexander;K. Murali-Krishna;Lisa. L. Lau;S. Southwood;J. Sidney;R. Chesnut;M. Matloubian;R. Ahmed
DOI：
10.1073/pnas.93.17.9102
发表时间：
1996-08-20
影响因子：
11.1
作者：
Zal, T;Weiss, S;Stockinger, B
通讯作者：
Stockinger, B
DOI：
10.1073/pnas.94.18.9848
发表时间：
1997-09-02
影响因子：
11.1
作者：
Pantaleo, G;Soudeyns, H;Fauci, AS
通讯作者：
Fauci, AS
影响因子：
3.7
作者：
van der Most, RG;Murali-Krishna, K;Ahmed, R
通讯作者：
Ahmed, R