Unaltered radial maze performance and brain acetylcholine of the endothelial nitric oxide synthase knockout mouse
Unaltered radial maze performance and brain acetylcholine of the endothelial nitric oxide synthase knockout mouse
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DOI：
10.1016/s0306-4522(01)00382-7
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Huston, JP
中科院分区：
文献类型：
--
作者：
Dere, E;Frisch, C;Huston, JP
从以前的研究结果的内皮型一氧化氮合酶（eNOS）基因失活对负强化水迷宫性能的有益影响，我们问这种改善学习能力是否也持有一个积极的强化径向迷宫任务。不像它对水迷宫任务的有益效果。eNOS基因失活并不促进径向迷宫的表现。eNOS敲除小鼠（eNOS-/-）和野生型小鼠（eNOS+/+）在位置学习的几天内的习得表现没有差异。与遗传背景C57 BL/6品系相比，eNOS-/-小鼠在放射状迷宫的位置学习版本中显示轻微的工作记忆缺陷，eNOS+/+小鼠显示更严重的工作记忆缺陷。eNOS失活的可能差异效应，与Morris水迷宫和放射状迷宫任务之间的强化偶然性差异有关。行为策略的要求，或不同的情感和生理的伴随固有的两个任务进行了讨论。这些任务独特的特点可能会受到不同的影响，报告的焦虑和高血压的eNOS基因失活的影响。对不同脑结构中乙酰胆碱浓度的死后测定显示，eNOS-/-和eNOS+/+小鼠之间的乙酰胆碱和胆碱含量没有差异，但eNOS+/+小鼠的额叶皮层中的乙酰胆碱和胆碱含量高于C57 BL/6小鼠。我们的研究结果表明，学习和记忆能力的表型不应仅依赖于一种学习任务，但应包括采用消极和积极强化应急的任务，以允许关于啮齿动物品系之间学习能力差异的有效陈述。(C)2001年IBRO。由Elsevier Science Ltd.出版，版权所有。
Proceeding from previous findings of a beneficial effect of endothelial nitric oxide synthase (eNOS) gene inactivation on negatively reinforced water maze performance, we asked whether this improvement in place learning capacities also holds for a positively reinforced radial maze task. Unlike its beneficial effects on the water maze task. eNOS gene inactivation did not facilitate radial maze performance. The acquisition performance over the days of place learning did not differ between eNOS knockout (eNOS-/-) and wild-type mice (eNOS+/+). eNOS-/- mice displayed a slight and eNOS+/+ mice a more severe working memory deficit in the place learning version of the radial maze compared to the genetic background C57BL/6 strain. Possible differential effects of eNOS inactivation, related to differences in reinforcement contingencies between the Morris water maze and radial maze tasks. behavioral strategy requirements, or to different emotional and physiological concomitants inherent in the two tasks are discussed. These task-unique characteristics might be differentially affected by the reported anxiogenic and hypertensional effects of eNOS gene inactivation. Post-mortem determination of acetylcholine concentrations in diverse brain structures revealed that acetylcholine and choline contents were not different between eNOS-/- and eNOS+/+ mice, but were increased in eNOS+/+ mice compared to C57BL/6 mice in the frontal cortex.Our findings demonstrate that phenotyping of learning and memory capacities should not rely on one learning task only, but should include tasks employing both negative and positive reinforcement contingencies in order to allow valid statements regarding differences in learning capacities between rodent strains. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.