High Culturability of Bacteria in Commercially Available Personal Composters for Fed-batch Treatment of Household Biowaste
High Culturability of Bacteria in Commercially Available Personal Composters for Fed-batch Treatment of Household Biowaste
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用于家庭生物废物补料分批处理的市售个人堆肥器中细菌的高可培养性
DOI：
10.1264/jsme2.18.94
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发表时间：
2003
影响因子：
2.2
通讯作者：
A. Hiraishi
中科院分区：
文献类型：
--
作者：
T. Narihiro;Yosuke Yamanaka;A. Hiraishi
不同类型的商业上可获得的个人堆肥被设计用于重复的分批进料处理的垃圾操作超过一个月，以研究在该过程的稳定状态下的微生物种群。显微镜直接计数和活菌平板计数记录的数量级为1011每克干重的固体废物堆肥混合物（SCM），和居民细菌的可培养性估计为54%的平均值。高培养能力也通过SCM本身和从其板上回收的细菌菌落的比较醌谱来证明。这些结果表明，传统的培养方法的工作更好地研究微生物群落结构在流加堆肥过程中比在自然环境中。殖民地醌谱法在评价原位微生物种群的培养偏倚和可培养性方面具有很大的应用前景。
Different types of commercially available personal composters which were designed for repeated fed-batch treatment of garbage were operated for more than one month in order to study microbial populations at the steady state of this process. Both microscopic direct counts and viable plate counts of bacteria recorded had an order of magnitude of 1011 per g dry weight of solid waste-compost mixture (SCM), and the culturability of the resident bacteria was estimated to be 54% on average. High culturability was also demonstrated by comparative quinone profiling of the SCM itself and bacterial colonies recovered on the plates therefrom. These results suggest that conventional cultivation methods work much better for studying microbial community structure in the fed-batch composting process than in natural environments. The colony quinone profiling method may have great promise for the evaluation of culture bias and culturability of in situ microbial populations.