Effect of divalent cations on antiparallel G-quartet structure of d(G4T4C4)

Effect of divalent cations on antiparallel G-quartet structure of d(G4T4C4)
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DOI:
10.1016/s0014-5793(01)02416-4
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发表时间:
2001-05-11
期刊:
影响因子:
3.5
通讯作者:
Sugimoto, N
Sugimoto, N
中科院分区:
生物学3区
文献类型:
--
作者:
Miyoshi, D;Nakao, A;Sugimoto, N

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得到了d(G(4)T(4)G(4))与1 mM二价阳离子(Mg 2+、Ca 2+、Mn 2+、Co 2+和Zn 2+)形成反平行G-四重态的热力学参数。热力学参数表明,二价阳离子对d(G(4)T(4)G(4))的反平行G-四重态的不稳定性顺序为:Zn ~(2+)> Co ~(2+)> Mn ~(2+)> Mg ~(2+)> Ca ~(2+)。此外,较高浓度的二价阳离子诱导从antiparattel过渡到平行的G-四重结构。这些结果表明,这些二价阳离子是一个很好的工具,用于调节G-四重态结构。(C)2001年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
The thermodynamic parameters of an antiparallel G-quartet formation of d(G(4)T(4)G(4)) with 1 mM divalent cation (Mg2+, Ca2+, Mn2+, Co2+, and Zn2+) were obtained. The thermodynamic parameters showed that the divalent cation destabilizes the antiparallel G-quartet of d(G(4)T(4)G(4)) in the Following order: Zn2+ > Co2+ > Mn2+ > Mg2+ > Ca2+. In addition, a higher concentration of a divalent cation induced a transition from an antiparattel to a parallel G-quartet structure. These results indicate that these divalent cations are a good tool for regulating the G-quartet structures. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.