Multichannel diffuse optical Raman tomography for bone characterization in vivo: a phantom study.
Multichannel diffuse optical Raman tomography for bone characterization in vivo: a phantom study.
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DOI：
10.1364/boe.3.002299
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发表时间：
2012-09-01
影响因子：
3.4
通讯作者：
Morris MD
中科院分区：
文献类型：
--
作者：
Demers JL;Davis SC;Pogue BW;Morris MD
拉曼光谱用于收集骨组织的矿物质和有机成分的信息，以分析其组成。通过空间偏移拉曼光谱测量骨骼的拉曼信号，可以确定骨骼的健康状况。我们定制了一个具有8个收集通道的系统，这些通道由单独的光纤组成，并连接到单独的光谱仪和冷却CCD。该平行检测系统被用于扫描具有两种尺寸的Teflon夹杂物的明胶模体。使用通道特异性多项式拟合将拉曼信号与自发荧光背景解耦。使用基于模型的扩散层析成像方法恢复了具有高对比度背景比的拉曼产率和准确的空间分辨率的图像。
Raman spectroscopy is used to gather information on the mineral and organic components of bone tissue to analyze their composition. By measuring the Raman signal of bone through spatially offset Raman spectroscopy the health of the bone can be determined. We’ve customized a system with 8 collection channels that consist of individual fibers, which are coupled to separate spectrometers and cooled CCDs. This parallel detection system was used to scan gelatin phantoms with Teflon inclusions of two sizes. Raman signals were decoupled from the autofluorescence background using channel specific polynomial fitting. Images with high contrast to background ratios of Raman yield and accurate spatial resolution were recovered using a model-based diffuse tomography approach.