Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles
Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles
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DOI：
10.1126/science.287.5454.873
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发表时间：
2000-02-04
期刊：
影响因子：
56.9
通讯作者：
Friend, SH
中科院分区：
文献类型：
--
作者：
Roberts, CJ;Nelson, B;Friend, SH
全基因组转录谱被用来监测酵母信息素反应过程中的信号转导。遗传操作允许分析潜在的信息素信号，细胞周期控制和极化形态发生的基因表达的变化。一个二维的层次聚类矩阵，涵盖383个最高度调控的基因，从46个不同的实验条件构建。共表达基因的诊断子集反映了信号传导活性、串扰和多种丝裂原活化蛋白激酶（MAPK）通路的重叠。两种不同的MAPKs-Fus 3 p和Kss 1 p-指定的配置文件的分析显示丝状生长和交配反应的功能重叠。整体转录分析反映了与信号转导途径的激活和干扰相关的生物学反应。
Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs-Fus3p and Kss1p-revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.