Odorant-induced membrane potential depolarization of AIY interneuron in Caenorhabditis elegans
Odorant-induced membrane potential depolarization of AIY interneuron in Caenorhabditis elegans
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气味诱导的秀丽隐杆线虫 AIY 中间神经元膜电位去极化
DOI：
10.1016/j.neulet.2013.02.016
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发表时间：
2013
期刊：
影响因子：
2.5
通讯作者：
Oka K
中科院分区：
文献类型：
--
作者：
Shidara H;Kobayashi J;Tanamoto R;Hotta K;Oka K
C.尽管已经显示秀丽线虫具有不寻常的区域特异性Ca 2+动力学，但是由于技术限制，这些神经元的区域特异性Ca 2+和膜电位响应特性在很大程度上是未知的。在这份报告中，我们专注于这些神经元之一，AIY interneuron，在气味和温度刺激，以确定是否膜电位和Ca2+是区域特异性的动力学和彼此不同的，在那里钙离子动力学已被检测到只有在神经突，而不是索马。为了观察AIY的索马和神经突中的电压变化，我们使用电压敏感荧光蛋白（VSFP）2.42。首先，我们证实了传感器蛋白在C. elegans通过用高浓度KCl去极化AIY中间神经元。接下来，我们观察了气味（异戊醇）刺激过程中的神经突和索马的膜电位去极化。此外，直接应用高KCl的膜电位去极化诱导了索马体中Ca 2+的增加。从这些结果中，我们得出结论，膜电位行为和Ca2+动力学在AIY不同的亚细胞区域，VSFP 2.42可以是一个有用的工具，用于研究在单个神经元的信息处理。
Although some interneurons in C. elegans have been shown to have unusual region-specific Ca2+dynamics, the region-specific Ca2+and membrane potential response properties of these neurons are largely unknown due to technical limitations. In this report, we focused on one of these neurons, AIY interneuron, where Ca2+dynamics have been detected only in neurites, and not the soma, during odor and temperature stimulation to determine whether membrane potential and Ca2+are region-specific dynamics and distinct from one another. To visualize voltage change both in the soma and neurites of AIY, we used voltage-sensitive fluorescent protein (VSFP) 2.42. First, we confirmed that the sensor protein worked correctly in C. elegans by depolarizing AIY interneuron with high concentrations of KCl. Next, we observed membrane potential depolarization during odor (isoamyl alcohol) stimulation in both neurites and the soma. Additionally, depolarization of membrane potential with direct application of high KCl induced a Ca2+increase in the soma. From these results, we conclude that membrane potential behavior and Ca2+dynamics in AIY differ in its subcellular regions and that VSFP2.42 can be a useful tool for studying information processing in single neurons.