Identification of Siglec Cis-Ligands by Proximity Labeling
Identification of Siglec Cis-Ligands by Proximity Labeling
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通过邻近标记鉴定 Siglec 顺式配体
DOI：
10.1007/978-1-0716-0430-4_8
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发表时间：
2020
影响因子：
--
通讯作者：
Tsubata Takeshi
中科院分区：
文献类型：
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作者：
Alborzian Deh Sheikh Amin;Akatsu Chizuru;Tsubata Takeshi
已知Siglecs由显示特定唾液酸配体的膜分子结合和调节，并存在于同一细胞上（顺式配体）。由于Siglecs与糖聚糖配体的亲和力较低，免疫沉淀等常规方法不适合用于Siglecs顺式配体的鉴定。在这里，我们描述了CD22（也称为Siglec-2）的顺式配体在B淋巴细胞上通过使用酪酰胺的接近标记的高效和特异性标记。该方法也可用于其它Siglecs的顺式配体的标记。
Siglecs are known to be bound and regulated by membrane molecules that display specific sialic acid-containing ligands and are present on the same cell (cis-ligands). Because of the low-affinity binding of Siglecs to the glycan ligands, conventional methods such as immunoprecipitation are not suitable for identification of Siglec cis-ligands. Here we describe efficient and specific labeling of cis-ligands of CD22 (also known as Siglec-2) on B lymphocytes by proximity labeling using tyramide. This method may also be applicable to labeling of cis-ligands of other Siglecs.