Synergistic effect of low-intensity pulsed ultrasound on growth factor stimulation of nucleus pulposus cells
Synergistic effect of low-intensity pulsed ultrasound on growth factor stimulation of nucleus pulposus cells
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DOI：
10.1002/jor.20460
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发表时间：
2007-12-01
影响因子：
2.8
通讯作者：
Sakai, Daisuke
中科院分区：
文献类型：
--
作者：
Hiyama, Akihiko;Mochida, Joji;Sakai, Daisuke
低强度脉冲超声（LIPUS）已被报道刺激各种细胞的活性。我们已经报道了人椎间盘髓核细胞系合成蛋白聚糖（PG）的能力通过暴露于LIPUS而增加，并假设这种反应的机制之一是转化生长因子-P I型受体基因（TGF β R1）表达的增加。因此，进行本研究以评估LIPUS和TGF-β对从犬收集的髓核细胞的协同作用。将细胞在四组不同的条件下培养：对照组（A组）、LIPUS组（B组）、TGF-β 1组（Q组）和LIPUS + TGF-β 1组（D组）。通过测量细胞增殖、PG合成、PG含量、TGF β R1的基因表达和TGF-β 1浓度来评价它们。在培养过程中，增殖没有显着差异。然而，PG合成和内源性TGF-β 1的产生增加，并证明了LIPUS和TGF-β之间的协同效应。由于LIPUS是安全和非侵入性的，本研究的结果表明，这将是一个有前途的新疗法，用于预防椎间盘退变，这是说是腰痛的主要原因之一。(C)2007骨科研究学会。
Low-intensity pulsed ultrasound (LIPUS) has been reported to stimulate the activity of various cells. We have reported that the capacity of human intervertebral nucleus pulposus cell line to synthesize proteoglycan (PG) was increased by exposure to LIPUS, and postulated that one of the mechanisms underlying this response was an increase in expression of the transforming growth factor-P type I receptor gene (TGF beta R1). Therefore, the present study was conducted to assess the synergistic effect of LIPUS and TGF-beta on nucleus pulposus cells harvested from canines. The cells were cultured under four different sets of conditions: control group (Group A), LIPUS group (Group B), TGF-beta 1 group (Group Q, and LlPUS + TGF-beta 1 group (Group D). They were evaluated by measuring cell proliferation, PG synthesis, PG content, gene expression of TGF beta R1, and TGF-beta 1 concentration. There were no significant differences in proliferation during culture. However, PG synthesis and endogenous TGF-beta 1 production increased and demonstrated a synergistic effect between LIPUS and TGF-beta. Because LIPUS is safe and noninvasive, the results of the present study suggest that it would be a promising new therapy for prevention of intervertebral disc degeneration, which is said to be one of the primary causes of low back pain. (C) 2007 Orthopaedie Research Society.