Altered neutrophil apoptosis activity is reversed by melatonin in liver ischemia-reperfusion
Altered neutrophil apoptosis activity is reversed by melatonin in liver ischemia-reperfusion
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DOI：
10.1034/j.1600-079x.2003.t01-1-00031.x
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发表时间：
2003-05-01
影响因子：
10.3
通讯作者：
Chen, HM
中科院分区：
文献类型：
--
作者：
Chen, JC;Ng, CJ;Chen, HM
迟发性中性粒细胞凋亡被认为是全身炎症反应的机制。在此，我们研究了褪黑激素对人肝缺血再灌注中性粒细胞凋亡的影响。我们研究了7例因肝肿瘤接受选择性肝切除术的患者和10例因胆囊结石接受腹腔镜胆囊切除术的患者。抽取10毫升血液，分离并培养人中性粒细胞。流式细胞术检测神经细胞凋亡活性、CD 18表达和呼吸爆发活性。另一组中性粒细胞包括来自接受肝切除术的患者的中性粒细胞，其被分离并与褪黑激素一起孵育。肝切除术或腹腔镜胆囊切除术后患者的神经细胞凋亡较术前延迟。与腹腔镜胆囊切除术患者相比，肝切除术患者的细胞凋亡活性降低更严重。来自接受肝切除术或腹腔镜胆囊切除术的患者的中性粒细胞被功能性激活。褪黑素可逆转肝切除术后中性粒细胞凋亡的延迟过程，增强其凋亡活性。这项研究表明，褪黑激素增强中性粒细胞凋亡的患者接受肝切除术涉及缺血和再灌注的人肝脏。
Delayed neutrophil apoptosis has been implicated as the mechanism of the systemic inflammatory response. Herein, we examined the effect of melatonin on the neutrophil apoptosis in ischemia and reperfusion of the human liver. We studied seven patients receiving elective hepatectomy for liver tumor and ten patients receiving laparoscopic cholecystectomy for gallstones. Ten milli liters of blood was drawn isolation and incubation of the human neutrophils. Neutrophil apoptosis activity and CD18 expression and respiratory burst activity were assessed flow cytometrically. Another group of neutrophils included those from the patients receiving hepatectomy and isolated and incubated with melatonin. Neutrophil apoptosis is delayed from patients after hepatectomy or laparoscopic cholecystectomy when compared with that of the preoperative state. The decrease in the apoptosis activity is more severe in patients receiving hepatectomy as compared with those receiving laparoscopic cholecystectomy. Neutrophils from patients receiving hepatectomy or laparoscopic cholecystectomy are functionally activated. Melatonin can reverse the delayed process and enhance the apoptosis activity in neutrophils from patients receiving hepatectomy. This study demonstrates that melatonin enhances neutrophil apoptosis in patients receiving hepatectomy involving ischemia and reperfusion of the human liver.