Borrelia burgdorferi Co-Localizing with Amyloid Markers in Alzheimer's Disease Brain Tissues.
Borrelia burgdorferi Co-Localizing with Amyloid Markers in Alzheimer's Disease Brain Tissues.
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DOI：
10.3233/jad-215398
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Sapi E
中科院分区：
文献类型：
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作者：
Senejani AG;Maghsoudlou J;El-Zohiry D;Gaur G;Wawrzeniak K;Caravaglia C;Khatri VA;MacDonald A;Sapi E
细菌或病毒感染被假设为影响神经退行性疾病的病因。 本研究检查了莱姆病病原体伯氏疏螺旋体螺旋体在诊断为阿尔茨海默病（AD）或帕金森病患者的脑尸检组织中的潜在存在。 对年龄匹配的对照组患者的脑组织切片进行抗原和B的DNA存在评估。burgdorferi使用各种方法。使用免疫组织化学方法评价阳性疏螺旋体结构与生物膜和AD标志物如淀粉样蛋白和磷酸化tau（p-Tau）的共定位。 结果表明存在B。在AD病理学患者中，其中一人先前被诊断患有莱姆病。有趣的是，与螺旋体结构一起发现了大量具有已知生物膜标记物藻酸盐的疏螺旋体阳性聚集体沿着。我们的免疫组化数据还显示，疏螺旋体阳性聚集体与淀粉样蛋白和磷酸化tau标记物共定位。进一步证明了B的潜在关系。burgdorferi和淀粉样蛋白，我们用B感染了两种哺乳动物细胞系。这导致感染后两种细胞系中淀粉样蛋白-β和p-Tau蛋白的表达显著增加。 这些结果表明，B.在AD脑组织中，不仅在螺旋体中，而且在已知的抗生素抗性生物膜形式及其共定位的淀粉样蛋白标志物中，可以发现伯氏螺旋体。总之，本研究提供了B。Burgdorferi感染和生物膜形成、AD病理学和慢性神经退行性疾病。
Infections by bacterial or viral agents have been hypothesized to influence the etiology of neurodegenerative diseases. This study examined the potential presence of Borrelia burgdorferi spirochete, the causative agent of Lyme disease, in brain autopsy tissue of patients diagnosed with either Alzheimer’s (AD) or Parkinson’s diseases. Brain tissue sections from patients with age-matched controls were evaluated for antigen and DNA presence of B. burgdorferi using various methods. Positive Borrelia structures were evaluated for co-localization with biofilm and AD markers such as amyloid and phospho-tau (p-Tau) using immunohistochemical methods. The results showed the presence of B. burgdorferi antigen and DNA in patients with AD pathology and among those, one of them was previously diagnosed with Lyme disease. Interestingly, a significant number of Borrelia-positive aggregates with a known biofilm marker, alginate, were found along with the spirochetal structures. Our immunohistochemical data also showed that Borrelia-positive aggregates co-localized with amyloid and phospho-tau markers. To further prove the potential relationship of B. burgdorferi and amyloids, we infected two mammalian cell lines with B. burgdorferi which resulted in a significant increase in the expression of amyloid-β and p-Tau proteins in both cells lines post-infection. These results indicate that B. burgdorferi can be found in AD brain tissues, not just in spirochete but a known antibiotics resistant biofilm form, and its co-localized amyloid markers. In summary, this study provides evidence for a likely association between B. burgdorferi infections and biofilm formation, AD pathology, and chronic neurodegenerative diseases.