A GUIDE TO THE USE OF THE EXUDING-STYLET TECHNIQUE IN PHLOEM PHYSIOLOGY
A GUIDE TO THE USE OF THE EXUDING-STYLET TECHNIQUE IN PHLOEM PHYSIOLOGY
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DOI：
10.1007/bf00394567
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发表时间：
1984-01-01
期刊：
影响因子：
4.3
通讯作者：
FRAME, JM
中科院分区：
文献类型：
--
作者：
FISHER, DB;FRAME, JM
渗出探针的使用为筛管生理学的研究提供了相当大的希望。然而，主要是因为切割昆虫针的困难，该技术仅应用于少数植物物种。根据我们的经验，比较了从韧皮部取食昆虫（包括蚜虫、蚧虫和粉蚧）的分泌管针中获得筛管分泌物的可用方法。41种植物。测试了35种昆虫提供口针分泌物的能力。除了少数几个测试的植物物种上的管芯产生至少一些渗出物，但许多物种的成功率和渗出的持续时间是不令人满意的韧皮部运输的详细调查。植物物种显然是获得可靠的渗出管针的最重要因素，尽管所用昆虫物种的大小和植物的生理条件也是重要的变量。铠装鳞提供了一种简单可靠的渗出探针来源，但对于大多数实验目的来说是不切实际的。射频显微烧灼的蚜虫针基本上是最有效的手段切割针。使用市民波段无线电作为射频源，提供了构造显微烧灼装置的说明。
The use of exuding stylets holds considerable promise for the investigation of sieve-tube physiology. However, largely because of difficulties in cutting insect stylets, the technique has been applied to only a few plant species. Based on our experience, a comparison is made of the available means of obtaining sieve-tube exudate from the exuding stylets of phloem-feeding insects, including aphids, scale and mealybugs. Forty-one plant species .apprx. 35 insect species were tested for their ability to provide stylet exudate. Stylets on all but a few of the plant species tested yielded at least some exudate, but the success rate and duration of exudation on many species were unsatisfactory for detailed investigations of phloem transport. Plant species apparently is the most important factor for obtaining reliably exuding stylets, although the size of the insect species used and the physiological condition of the plant are also important variables. Armored scale provide a simple and reliable source of exuding stylets, but are impractical for most experimental purposes. Radio-frequency microcautery of aphid stylets was substantially the most effective means of cutting stylets. Instructions are provided for constructing a microcautery unit, using a citizen''s band radio as the radio-frequency source.