Two-photon excitation microscopy for the study of living cells and tissues.
Two-photon excitation microscopy for the study of living cells and tissues.
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DOI：
10.1002/0471143030.cb0411s59
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发表时间：
2013-06-01
影响因子：
--
通讯作者：
Piston, David W
中科院分区：
文献类型：
--
作者：
Benninger, Richard K P;Piston, David W
Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages for three-dimensional and deep tissue imaging. This unit will describe the basic physical principles behind two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The principal advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate highly localized photochemistry in thick samples. Practical considerations for the application of two-photon microscopy will then be discussed, including recent technological advances. This unit will conclude with some recent applications of two-photon microscopy that highlight the key advantages over confocal microscopy and the types of experiments which would benefit most from its application.
