Target-triggered entropy-driven amplification system-templated silver nanoclusters for multiplexed microRNA analysis
Target-triggered entropy-driven amplification system-templated silver nanoclusters for multiplexed microRNA analysis
复制标题
用于多重 microRNA 分析的目标触发熵驱动放大系统模板银纳米簇
DOI：
10.1016/j.bios.2020.112757
复制
发表时间：
2021-01-15
影响因子：
12.6
通讯作者：
Qiu, Feng
中科院分区：
文献类型：
--
作者：
Li, Fengyun;Li, Gen;Qiu, Feng
microRNA（miRNAs）是人类疾病诊断、预后和治疗的重要生物标志物。同时敏感和选择性地检测多种miRNA将极大地促进癌症的早期和准确诊断。本研究开发了一种新的熵驱动扩增系统-模板化银纳米簇传感平台，用于肿瘤相关miRNA的多重分析。该传感平台由靶触发的熵驱动催化与发光可调的DNA模板银纳米团簇（Ag NCs）耦合而成。在靶miRNA存在下，传感平台启动复合物的分支迁移和链置换，该复合物具有用于稳定发光Ag NC的六碱基胞嘧啶环。目标是循环产生的新的催化，同时关闭银NC的荧光;这是伴随着显着放大的光学读出。在这项研究中，提出了两种不同的复合物稳定的银纳米粒子系统，黄色发光的银纳米粒子和红色发光的银纳米粒子生物传感器，使分析的miRNA-141和miRNA-155的检测限分别为6.1 pM和8.7 pM。由于该平台具有良好的选择性、灵活性和窄带激发特性，在缓冲液、生物细胞裂解液和人血清样品中实现了miRNA-141和miRNA-155的多重同步检测，结果令人满意。该方法简单、灵活、方便，为多生物标志物分析及相关临床应用提供了有力的工具。
MicroRNAs (miRNAs) are important biomarkers for the diagnosis, prognosis, and treatment of human diseases. Sensitive and selective detection of multiple miRNAs simultaneously will greatly facilitate the early and accurate diagnosis of cancers. Herein, a novel entropy-driven amplification system-templated silver nanoclusters sensing platform was developed for the multiplexed analysis of tumor-associated miRNAs. The sensing platform was constructed by coupling target-triggered entropy-driven catalysis with luminescence adjustable DNA-templated silver nanoclusters (Ag NCs). In the presence of target miRNA, the sensing platform initiates the branch migration and strand displacement of the complex, which has a six-base cytosine loop for stabilizing the luminous Ag NCs. The target is cyclically generated for new catalysis while turning off the fluorescence of Ag NCs; this is accompanied by a significantly amplified optical readout. In this study, two different complex-stabilized Ag NCs systems were proposed, the yellow-emitting Ag NCs and red-emitting Ag NCs biosensors enabled the analysis of miRNA-141 and miRNA-155 with detection limits of 6.1 pM and 8.7 pM, respectively. Impressively, owing to the excellent selectivity, flexibility, and narrow-band excitation of the platform, the multiplexed synchronous detection of miRNA-141 and miRNA-155 were achieved in buffer, biological cell lysates and human serum samples with satisfactory results. The simple, flexible, and convenient strategy provides a powerful tool for multiple biomarkers analysis and related clinical applications.