Anaeromyxobacter oryzae sp. nov., Anaeromyxobacter diazotrophicus sp. nov. and Anaeromyxobacter paludicola sp. nov., isolated from paddy soils
Anaeromyxobacter oryzae sp. nov., Anaeromyxobacter diazotrophicus sp. nov. and Anaeromyxobacter paludicola sp. nov., isolated from paddy soils
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米厌氧粘杆菌 sp.
DOI：
10.1099/ijsem.0.005546
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发表时间：
2022
影响因子：
2.8
通讯作者：
Senoo Keishi
中科院分区：
文献类型：
--
作者：
Itoh Hideomi;Xu Zhenxing;Mise Kazumori;Masuda Yoko;Ushijima Natsumi;Hayakawa Chie;Shiratori Yutaka;Senoo Keishi
从日本水稻土中分离到3株细菌（Red 232 T、Red 267 T和Red 630 T）。这些菌株的细胞呈革兰氏染色阴性，兼性厌氧，长棒状，具有单毛鞭毛或绒毛状结构，可运动，在琼脂平板上形成红色菌落。基于16 S rRNA基因和多个单拷贝基因序列构建的系统发育树显示，3株菌与厌氧黏细菌属的模式菌株聚为一簇，独立于其他菌株属。3株分离菌株与Anaeromyxobacter模式菌株Anaeromyxobacter dehaloans 2CP-1 T的16 S rRNA基因序列和基因组相似度分别为95.4-97.4%，平均核苷酸同一性为75.3-79.5%，数字DNA-DNA杂交为19.6-21.7%，平均氨基酸同一性为64.1-72.6%，所有这些都低于物种划分阈值。固氮酶基因在3个新菌株的基因组中均有表达，但在A.脱卤剂2CP-1 T。此外，多个基因组，生理和化学分类特征支持这三个菌株之间的区别。根据本研究中的证据，这三个分离株代表了三个新的独立物种，并建议将其命名为：Anaeromyxamericoryzaesp。十一月，固氮厌氧黏菌nov.和Anaeromyxapoladicolasp.模式菌株分别为Red 232 T（=NBRC 114074 T =MCCC 1 K 03954 T）、Red 267 T（=NBRC 114075 T =MCCC 1 K 04211 T）和Red 630 T（=NBRC 114076 T =MCCC 1 K 03957 T）。
Three bacterial strains (Red232T, Red267Tand Red630T) were isolated from paddy soils sampled in Japan. Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates. Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains ofAnaeromyxobacterspecies, independent from any other strain genera. Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain ofAnaeromyxobacter,Anaeromyxobacter dehalogenans2CP-1T, were 95.4–97.4% for 16S rRNA gene sequence, 75.3–79.5% for average nucleotide identity, 19.6–21.7% for digital DNA–DNA hybridization and 64.1–72.6% for average amino acid identity, all of which are below the species delineation thresholds. Nitrogenase genes were observed in the genomes of the three novel strains, but not inA. dehalogenans2CP-1T. Moreover, multiple genomic, physiological and chemotaxonomic features supported the discrimination between these three strains. Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed:Anaeromyxobacter oryzaesp. nov.,Anaeromyxobacter diazotrophicussp. nov. andAnaeromyxobacter paludicolasp. nov. The type strains are Red232T(=NBRC 114074T=MCCC 1K03954T), Red267T(=NBRC 114075T=MCCC 1K04211T), and Red630T(=NBRC 114076T=MCCC 1K03957T), respectively.