AN IN-VITRO SYSTEM FOR ADHESION AND FUSION OF MAIZE GAMETES
AN IN-VITRO SYSTEM FOR ADHESION AND FUSION OF MAIZE GAMETES
复制标题
DOI：
10.1126/science.263.5153.1598
复制
发表时间：
1994-03-18
期刊：
影响因子：
56.9
通讯作者：
DUMAS, C
中科院分区：
文献类型：
--
作者：
FAURE, JE;DIGONNET, C;DUMAS, C
开花植物体外受精系统的发展对于理解和控制受精机制非常重要。这里描述了一种分离的玉米配子融合的方法。在含有 5 mM 氯化钙的培养基中，精子和卵细胞粘附几分钟，然后在 10 秒内融合。该方法专门针对雄性-雌性配子对，可实现 80% 的融合，而与配子和叶肉细胞的其他组合的融合频率较低。体外受精的卵子不会与额外的雄配子融合，这表明存在多精受精的障碍。
The development of in vitro fertilization systems in flowering plants is important for understanding and controlling the mechanisms of fertilization. Here a method is described in which isolated maize gametes fuse. In a medium containing 5 mM calcium chloride, sperm and egg cells adhere for several minutes and then fuse within 10 seconds. The method is specific to male-female gamete pairs and results in 80 percent fusion, whereas fusions with other combinations of gametic and mesophyllic cells are less frequent. Eggs fertilized in vitro do not fuse with additional male gametes, which suggests that a block to polyspermy exists.