EFFICIENT METHODS FOR ATTACHING NONRADIOACTIVE LABELS TO THE 5' ENDS OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES
EFFICIENT METHODS FOR ATTACHING NONRADIOACTIVE LABELS TO THE 5' ENDS OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES
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DOI：
10.1093/nar/14.15.6227
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发表时间：
1986-08-11
影响因子：
14.9
通讯作者：
GAIT, MJ
中科院分区：
文献类型：
--
作者：
AGRAWAL, S;CHRISTODOULOU, C;GAIT, MJ
描述了两种类型的接头分子的合成，这些接头分子可用于将非放射性标记（例如生物素和荧光团）特异性附着到合成寡脱氧核糖核苷酸的 5' 末端。连接子的设计使得它们可以与寡核苷酸偶联，作为使用商业 DNA 合成机器进行固相合成的最后一步。使用抗生物素杂交瘤/过氧化物酶检测系统可以提高生物素检测的灵敏度。
The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5′ terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.