Cultivation and Laboratory Maintenance of Chlamydia trachomatis.
Cultivation and Laboratory Maintenance of Chlamydia trachomatis.
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DOI：
10.1002/9780471729259.mc11a01s00
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发表时间：
2005-07-01
影响因子：
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通讯作者：
Scidmore, Marci A
中科院分区：
文献类型：
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作者：
Scidmore, Marci A
衣原体属是专性细胞内细菌，需要在哺乳动物细胞内生长以繁殖和存活。因此，衣原体不能在常规细菌培养基上生长。这种特性使得衣原体很难在实验室中生长和维持。直到1965年，鸡胚卵黄囊中的传代是分离和繁殖该生物体的唯一方法。从那时起，一个组织培养系统已经可以使衣原体的实验室培养更容易。然而，除了LGV血清型，大多数C。沙眼菌株不容易感染组织培养细胞。使用化学或机械辅助来增加它们的传染性。今天，大量的感染性生物体可以通过感染细胞裂解物的Renografin密度梯度离心来纯化。C.沙眼衣原体的实验室研究，大大提高了对沙眼衣原体发病机制的认识。沙眼病原体
Chlamydia species are obligate intracellular bacteria that require growth inside mammalian cells for propagation and survival. As a result, Chlamydia cannot be grown on conventional bacteriological medium. This property makes Chlamydia difficult organisms to grow and maintain in the laboratory. Up until 1965, passage in the yolk sac of the embryonated hen egg was the only way to isolate and propagate the organism. Since then, a tissue culture system has been available that allows easier laboratory culture of the Chlamydia species. However, with the exception of the LGV serovars, most C. trachomatis strains do not readily infect tissue culture cells. Chemical or mechanical assistance is used to increase their infectivity. Today, large numbers of infectious organisms can be purified through Renografin density gradient centrifugation of infected cell lysates. The ability to propagate C. trachomatis in the laboratory has greatly increased the understanding of the pathogenesis of C. trachomatis organisms.