Compatibility and competitiveness of a laboratory strain of Anastrepha fraterculus (Diptera: Tephritidae) after irradiation treatment
Compatibility and competitiveness of a laboratory strain of Anastrepha fraterculus (Diptera: Tephritidae) after irradiation treatment
复制标题
DOI：
10.1653/0015-4040(2007)90
复制
发表时间：
2007-03-01
影响因子：
1.4
通讯作者：
Vilardi, Juan Cesar
中科院分区：
文献类型：
--
作者：
Allinghi, Armando;Calcagno, Graciela;Vilardi, Juan Cesar
我们在半自然田间笼养条件下评估了实验室品系（LAB）与南美按实蝇（Anastrepha fraterculus (Wiedemann)）野生种群（TUC）的性兼容性和竞争力。LAB品系是在奥比斯波·科隆布雷斯农业工业实验站（阿根廷图库曼）的半大规模饲养条件下培育的。野生果蝇是从阿根廷图库曼的霍尔科·莫勒受侵染的番石榴果实中获取的。LAB蛹在成虫羽化前48小时接受（钴 - 60）辐射。所测试的剂量为0（对照）、40、70和100戈瑞。将25只TUC雄性和25只TUC雌性以及25只LAB雄性和25只LAB雌性分别放入笼子中，并收集交配对。每次只考虑一种辐射剂量。将雌性分开，让它们在人工果实中产卵，以根据相应的孵化率估算诱导不育性。交配开始时间在品系之间以及辐射处理之间没有显著差异。交配持续时间在辐射剂量之间显示出极显著差异，但在品系之间没有差异。性隔离指数（ISI）和相对不育指数（RSI）表明LAB和TUC完全兼容，TUC和LAB的雄性在交配竞争力方面没有差异，并且所测试范围内的辐射没有影响这些指数。未受辐射的LAB雌性比TUC雌性表现出更高的交配倾向。然而，随着辐射剂量的增加，观察到雌性相对表现指数（FRPI）显著降低。对诱导不育性的分析表明，40戈瑞的处理使雄性生育力从约80%降低到0.75%，更高剂量则导致完全不育。在雌性中，40戈瑞剂量使生育力降低到约2%，更高剂量则阻止产卵。
We evaluated under semi-natural field cage conditions sexual compatibility and competitiveness of a laboratory strain (LAB) compared to a wild population (TUC) of Anastrepha fraterculus (Wiedemann). The LAB strain is produced under semi-mass rearing conditions at the Estacion Experimental Agroindustrial Obispo Colombres facility (Tucuman, Argentina). Wild flies were obtained at Horco Molle (Tucuman, Argentina) from infested guava fruits. LAB pupae were irradiated (Co-60) 48 h before adult emergence. The tested doses were 0 (control), 40, 70, and 100 Gy. Twenty-five males and 25 females each of TUC and LAB were released into cages and mating pairs collected. Only 1 irradiation dose was considered at a time. Females were separated and allowed to lay eggs into artificial fruits to estimate induced sterility from the corresponding hatching rate. Copulation start time did not differ Significantly between strains nor among irradiation treatments. Copulation duration showed highly significant differences among irradiation doses, but no differences between strains. The index of sexual isolation (ISI) and the relative sterility index (RSI) indices indicated that LAB and TUC are fully compatible, males from TUC and LAB did not differ in mating competitiveness, and irradiation within the range tested did not affect these indices. Non-irradiated LAB females exhibited higher mating propensity than TUC ones. However, a significant reduction in the female relative performance index (FRPI) index was observed with increasing irradiation dose. The analysis of induced sterility indicated that treatment with 40 Gy reduces male fertility from about 80% to 0.75%, and higher doses produce total sterility. In females, the 40 Gy dose reduces fertility to about 2% and higher doses prevent egg laying.