Viral-induced self-assembly of magnetic nanoparticles allows the detection of viral particles in biological media
Viral-induced self-assembly of magnetic nanoparticles allows the detection of viral particles in biological media
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DOI：
10.1021/ja036409g
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发表时间：
2003-08-27
影响因子：
15
通讯作者：
Weissleder, R
中科院分区：
文献类型：
--
作者：
Perez, JM;Simeone, FJ;Weissleder, R
与病毒表面特异性抗体缀合的单分散磁性纳米颗粒在特异性病毒颗粒存在下自组装以产生具有增强的磁性的超分子结构，如通过磁共振方法（NMR/MRI）检测的。所观察到的磁弛豫变化，发生在病毒诱导的组装允许在复杂的生物介质中的病毒的高灵敏度和选择性检测。所开发的方法显示出特异性检测浓度为5个病毒颗粒/10 μL的腺病毒-5和单纯疱疹病毒-1，而不需要大量的样品制备。这种新方法的应用范围从生物样品中病毒的高通量NMR检测到体内病毒分布的潜在MR成像。
Monodisperse magnetic nanoparticles conjugated with virus-surface-specific antibodies self-assemble in the presence of specific viral particles to create supramolecular structures with enhanced magnetic properties, as detected by magnetic resonance methods (NMR/MRI). The observed magnetic relaxation changes that occur upon viral-induced assembly allowed for highly sensitive and selective detection of a virus in complex biological media. The developed method was shown to specifically detect adenovirus-5 and herpes simplex virus-1 at concentrations of 5 viral particles/10 μL without the need of extensive sample preparation. The applications of this new method span from high-throughput NMR detection of viruses in biological samples to potential MR imaging of viral distribution in vivo.