Hemopoietic stromal precursors in long-term culture of bone marrow: I. Precursor characteristics, kinetics in culture, and dependence on quality of donor hemopoietic cells in chimeras.
Hemopoietic stromal precursors in long-term culture of bone marrow: I. Precursor characteristics, kinetics in culture, and dependence on quality of donor hemopoietic cells in chimeras.
复制标题
骨髓长期培养中的造血基质前体：I.前体特征、培养动力学以及嵌合体中对供体造血细胞质量的依赖性。
作者：
J. Chertkov;N. Drize;O. Gurevitch;G. Udalov
长期Dexter型骨髓培养的贴壁细胞层含有造血基质前体，能够在体内移植时转移造血微环境。异位造血的病灶在维持造血的特性上与正常骨髓相对应。培养基质前体的放射敏感性与前培养敏感性无差异。基质前体在培养5-6周后失去成骨潜能，但能够产生稳定的异位造血灶，由多达10(8)个有核造血细胞组成。这些结果证明骨不是异位灶造血环境的重要组成部分。在嵌合体中，暴露于等剂量的辐射，但由新鲜分离或反复传代的骨髓重建，当使用自我维持能力减弱的造血细胞时，基质前体的含量较低。这些数据表明造血基质前体细胞至少存在两个水平的前体细胞分化。
The adherent cell layer of long-term Dexter type bone marrow culture contains hemopoietic stromal precursors capable of transferring the hemopoietic microenvironment on transplantation in vivo. The foci of ectopic hemopoiesis that are created correspond to normal bone marrow in the character of the maintained hemopoiesis. Radiosensitivity of the cultured stromal precursors does not differ from preculture sensitivity. Stromal precursors lose osteogenic potential after 5-6 wk in culture, but are capable of creating stable foci of ectopic hemopoiesis composed of up to 10(8) nucleated hemopoietic cells. These results prove that bone is not an essential component of the hemopoietic environment in ectopic foci. In chimeras exposed to equal doses of radiation but reconstituted by freshly isolated or repeatedly passaged bone marrow, the content of stromal precursors is lower when hemopoietic cells of diminished self-maintenance are used. These data suggest the existence of a hemopoietic stromal precursor hierarchy with at least two levels of precursor differentiation.