Sulfonated Pyrene as a Photoregulator for Single-Stranded DNA Looping
Sulfonated Pyrene as a Photoregulator for Single-Stranded DNA Looping
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磺化芘作为单链 DNA 环的光调节剂
DOI：
10.1002/chem.202000184
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Sachiko Tojo and Kiyohiko Kawai
中科院分区：
文献类型：
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作者：
Jie Xu;Shunichi Miyamoto;Sachiko Tojo and Kiyohiko Kawai
通过光调节剂控制生物分子的构象和功能作为一种时空可控的疾病控制工具具有很大的前景。此外，将光调节剂引入生物分子也在构建智能纳米材料中得到了应用。尽管在过去的几年里取得了惊人的进展，但在生理条件下实现对生物分子构象和功能的高度可控和有效调控仍然是一个挑战。本文合成了磺化吡啶吡啶并将其作为光调节剂用于控制水溶液中单链dna （ssdna）的环。由于它的水溶性，在研究水溶液中的生物分子方面具有很大的优势。双重spy修饰的ssdna的环受辐射刺激并受spy调控。光电离产生自由基阳离子SPy（SPy.+）。spy协会。+与它的中性对应物SPy形成SPy的二聚自由基阳离子（SPy2.+）。在缔合过程中，稳定能释放形成spy2。+为ssdna的循环提供了驱动力。相反，形成的环状构象被spy2的形成所困住。+，这允许研究循环动力学。本文报道的结果表明，在生理条件下，spya可能作为光调节剂控制生物分子的构象和功能。
Controlling the conformation and function of biomolecules through photoregulators holds great promise as a spatiotemporally controllable tool for disease control. In addition, introducing photoregulators into biomolecules has also found applications in constructing smart nanomaterials. In spite of the astonishing advances that have been made in the past few years, realizing highly controllable and efficient regulation over the conformation and function of biomolecules under physiological conditions is still challenging. Herein, sulfonated pyreneSPywas synthesized and used as a photoregulator to control the looping of single‐stranded DNAs (ssDNAs) in aqueous solution. Due to its water solubility,SPymerits use in the study of biomolecules in aqueous solution. The looping of the doublySPy‐modified ssDNAs is stimulated by irradiation and regulated bySPy. Photoionization generates the radical cation ofSPy(SPy.+). The association ofSPy.+with its neutral counterpart,SPy, gives rise to the dimer radical cation ofSPy(SPy2.+). During the association process, the stabilization energy released to formSPy2.+provides a driving force for the looping of ssDNAs. Conversely, the formed loop conformations were trapped by the formation ofSPy2.+, and this allowed the looping dynamics to be investigated. The results reported herein suggest potential ofSPyas a photoregulator for controlling the conformation and function of biomolecules under physiological conditions.