Enzymes adsorbed on an ion exchanger as a post-column reactor: application to acetylcholine measurement.
Enzymes adsorbed on an ion exchanger as a post-column reactor: application to acetylcholine measurement.
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作为柱后反应器的离子交换器上吸附的酶：在乙酰胆碱测量中的应用。
DOI：
10.1016/s0021-9673(01)91673-5
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
C. Eva
中科院分区：
文献类型：
--
作者：
J. Meek;C. Eva
酶可用于高效液相色谱柱后反应器，通过将某些化合物转化为易于检测的产物来提高检测的灵敏度和特异性。酶可以共价结合到柱后反应器上，但更简单的方法是将它们结合到离子交换器或疏水相互作用载体上。利用胆碱氧化酶和胆碱酯酶在3厘米长的商用弱阴离子交换柱上的吸附，将该技术应用于乙酰胆碱的电化学检测。乙酰胆碱到过氧化氢的转化是定量的，在试剂盒中停留10秒。当使用低离子强度流动相时，从色谱柱上的酶洗脱可以忽略不计。新鲜的酶只需要每隔1-2周添加到试剂盒中。
Enzymes can be used in high-performance liquid chromatography post-column reactors to improve sensitivity and specificity of detection for some compounds by converting the compounds to easily detectable products. The enzymes can be covalently bound to a post-column reactor, but a simpler approach is to bind them by adsoption to an ion exchanger or a hydrophobic interaction support. This technique has been applied to electrochemical detection of acetylcholine by using adsorption of choline oxidase and cholinesterase to a 3-cm long commercially available weak anion-exchange cartridge. Conversion of acetylcholine to peroxide is quantitative during the 10-sec residence time in the cartridge. Enzyme elution from the cartridge is negligible when low ionic strength mobile phases are used. Fresh enzyme needs to be added to the cartridge at only 1–2 week intervals.